Match!

IDN2 Interacts with RPA and Facilitates DNA Double-Strand Break Repair by Homologous Recombination in Arabidopsis

Published on Mar 1, 2017in The Plant Cell8.631
· DOI :10.1105/tpc.16.00769
Mingming Liu1
Estimated H-index: 1
(PKU: Peking University),
Zhaoqing Ba7
Estimated H-index: 7
(THU: Tsinghua University)
+ 7 AuthorsYijun Qi44
Estimated H-index: 44
(THU: Tsinghua University)
Abstract
Repair of DNA double-strand breaks (DSBs) is critical for the maintenance of genome integrity. We previously showed that DSB-induced small RNAs (diRNAs) facilitate homologous recombination (HR)-mediated DSB repair in Arabidopsis thaliana. Here we show that INVOLVED IN DE NOVO 2 (IDN2), a double-stranded RNA (dsRNA) binding protein involved in small RNA-directed DNA methylation, is required for DSB repair in Arabidopsis. We find that IDN2 interacts with the heterotrimeric replication protein A (RPA) complex. Depletion of IDN2 or the diRNA-binding ARGONAUTE 2 (AGO2) leads to increased accumulation of RPA at DSB sites and mislocalization of the recombination factor RAD51. These findings support a model in which IDN2 interacts with RPA and facilitates the release of RPA from ssDNA tails and subsequent recruitment of RAD51 at DSB sites to promote DSB repair.
  • References (44)
  • Citations (4)
📖 Papers frequently viewed together
37 Citations
31 Citations
114 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
References44
Newest
#1Jered M. Wendte (IU: Indiana University)H-Index: 3
#2Craig S. Pikaard (IU: Indiana University)H-Index: 62
Abstract RNA-directed chromatin modification that includes cytosine methylation silences transposable elements in both plants and mammals, contributing to genome defense and stability. In Arabidopsis thaliana , most RNA-directed DNA methylation (RdDM) is guided by small RNAs derived from double-stranded precursors synthesized at cytosine-methylated loci by nuclear multisubunit RNA Polymerase IV (Pol IV), in close partnership with the RNA-dependent RNA polymerase, RDR2. These small RNAs help keep...
57 CitationsSource
#1Xiaofeng Fang (THU: Tsinghua University)H-Index: 6
#2Yijun Qi (THU: Tsinghua University)H-Index: 44
Argonaute (AGO) family proteins are effectors of RNAi in eukaryotes. AGOs bind small RNAs and use them as guides to silence target genes or transposable elements at the transcriptional or posttranscriptional level. Eukaryotic AGO proteins share common structural and biochemical properties and function through conserved core mechanisms in RNAi pathways, yet plant AGOs have evolved specialized and diversified functions. This Review covers the general features of AGO proteins and highlights recent ...
82 CitationsSource
#1Wenjing Qi (Northeast Normal University)H-Index: 2
#2Ruoxi Wang (Northeast Normal University)H-Index: 6
Last. Xianlu Zeng (Northeast Normal University)H-Index: 20
view all 9 authors...
37 CitationsSource
#1Ran Chen (Roy J. and Lucille A. Carver College of Medicine)H-Index: 3
#2Marc S. Wold (Roy J. and Lucille A. Carver College of Medicine)H-Index: 46
Replication Protein A (RPA), the major single-stranded DNA-binding protein in eukaryotic cells, is required for processing of single-stranded DNA (ssDNA) intermediates found in replication, repair and recombination. Recent studies have shown that RPA binding to ssDNA is highly dynamic and that more than high-affinity binding is needed for function. Analysis of DNA binding mutants identified forms of RPA with reduced affinity for ssDNA that are fully active, and other mutants with higher affinity...
82 CitationsSource
#1Verena Eschbach (KIT: Karlsruhe Institute of Technology)H-Index: 1
#2Daniela Kobbe (KIT: Karlsruhe Institute of Technology)H-Index: 9
The heterotrimeric RPA (replication protein A) protein complex has single-stranded DNA-binding functions that are important for all DNA processing pathways in eukaryotic cells. In Arabidopsis thaliana, which has five homologs of the RPA1 subunit and two homologs each of RPA2 and RPA3, in theory 20 RPA complexes could form. Using Escherichia coli as a heterologous expression system and analysing the results of the co-purification of the different subunits, we conclude that AtRPA1a interacts with ...
8 CitationsSource
#1Marjori Matzke (AS: Academia Sinica)H-Index: 61
#2Rebecca A. MosherH-Index: 14
RNA-directed DNA methylation (RdDM) is a major mechanism of transcriptional gene silencing in plants that is mediated through small interfering RNAs (siRNAs) and plant-specific RNA polymerases. This Review summarizes our latest understanding of RdDM, including its genomic targets, molecular mechanisms (such as emerging non-canonical pathways) and diverse biological roles.
555 CitationsSource
#1Min Gao (CAS: Chinese Academy of Sciences)H-Index: 9
#2Wei Wei (THU: Tsinghua University)H-Index: 2
Last. Jannie Rendtlew Danielsen (CAS: Chinese Academy of Sciences)H-Index: 10
view all 19 authors...
DNA double-strand breaks (DSBs) are highly cytotoxic lesions and pose a major threat to genome stability if not properly repaired. We and others have previously shown that a class of DSB-induced small RNAs (diRNAs) is produced from sequences around DSB sites. DiRNAs are associated with Argonaute (Ago) proteins and play an important role in DSB repair, though the mechanism through which they act remains unclear. Here, we report that the role of diRNAs in DSB repair is restricted to repair by homo...
92 CitationsSource
#1Behailu B. Aklilu (UNH: University of New Hampshire)H-Index: 3
#2Ryan S. Soderquist (UNH: University of New Hampshire)H-Index: 1
Last. Kevin M. Culligan (UNH: University of New Hampshire)H-Index: 13
view all 3 authors...
Replication Protein A (RPA) is a heterotrimeric protein complex that binds single-stranded DNA. In plants, multiple genes encode the three RPA subunits (RPA1, RPA2 and RPA3), including five RPA1-like genes in Arabidopsis. Phylogenetic analysis suggests two distinct groups composed of RPA1A, RPA1C, RPA1E (ACE group) and RPA1B, RPA1D (BD group). ACE-group members are transcriptionally induced by ionizing radiation, while BD-group members show higher basal transcription and are not induced by ioniz...
37 CitationsSource
#1Koichiro Tamura (Tokyo Metropolitan University)H-Index: 31
#2Glen Stecher (ASU: Arizona State University)H-Index: 8
Last. Sudhir Kumar (ASU: Arizona State University)H-Index: 67
view all 5 authors...
We announce the release of an advanced version of the Molecular Evolutionary Genetics Analysis (MEGA) software, which currently contains facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis. In version 6.0, MEGA now enables the inference of timetrees, as it implements the RelTime method for estimating divergence times for all branching points in a phylogeny. A new Timetree Wizard in MEGA6 facilitates this timetree inference...
24.3k CitationsSource
#1Yongyou Zhu (UM: University of Michigan)H-Index: 2
#2M. Jordan Rowley (UM: University of Michigan)H-Index: 16
Last. Andrzej T. Wierzbicki (UM: University of Michigan)H-Index: 19
view all 4 authors...
RNA-mediated transcriptional silencing prevents deleterious effects of transposon activity and controls the expression of protein-coding genes. It involves long noncoding RNAs (lncRNAs). In Arabidopsis thaliana, some of those lncRNAs are produced by a specialized RNA Polymerase V (Pol V). The mechanism by which lncRNAs affect chromatin structure and mRNA production remains mostly unknown. Here we identify the SWI/SNF ATP-dependent nucleosome-remodeling complex as a component of the RNA-mediated ...
118 CitationsSource
Cited By4
Newest
#1Tien Van Vu (Gyeongsang National University)H-Index: 2
#2Yeon Woo Sung (Gyeongsang National University)H-Index: 2
Last. Jae-Yean Kim (Gyeongsang National University)H-Index: 2
view all 6 authors...
Continuing crop domestication/redomestication and modification is a key determinant of the adaptation and fulfillment of the food requirements of an exploding global population under increasingly challenging conditions such as climate change and the reduction in arable lands. Monocotyledonous crops are not only responsible for approximately 70% of total global crop production, indicating their important roles in human life, but also the first crops to be challenged with the abovementioned hurdle...
2 CitationsSource
#1Gwonjin Lee (RUB: Ruhr University Bochum)H-Index: 1
#2Julia Quintana (RUB: Ruhr University Bochum)H-Index: 1
Last. Ute Kraemer (RUB: Ruhr University Bochum)H-Index: 2
view all 7 authors...
Heavy metal-rich toxic soils and ordinary soils are both natural habitats of Arabidopsis halleri, different from closely related plant species including A. thaliana. Here we demonstrate enhanced Cd hypertolerance and attenuated Cd accumulation in plants originating from the most highly heavy metal-contaminated A. halleri site in Europe at Ponte Nossa (Noss/IT), compared to A. halleri from non-metalliferous (NM) sites at a small and a larger phylogenetic distance. In the two populations from NM s...
1 CitationsSource
#1Takeshi Hirakawa (Ridai: Tokyo University of Science)H-Index: 5
#2Sachihiro Matsunaga (Ridai: Tokyo University of Science)H-Index: 38
As a sessile organism, plants are constantly challenged by diverse environmental stresses that threaten genome integrity by way of induction of DNA damage. In plants, each tissue is composed of differentiated cell types and the response to DNA damage differs among each cell type. However, limited information is available on the DNA repair activity of different cell types in response to DNA damage in plants. A chromatin remodeling factor RAD54, which plays an important role in alteration of chrom...
1 CitationsSource
#1Jin-Hong KimH-Index: 11
#2Tae Ho RyuH-Index: 3
Last. Byung Yeoup ChungH-Index: 18
view all 5 authors...
Abstract Plants orchestrate various DNA damage responses (DDRs) to overcome the deleterious impacts of genotoxic agents on genetic materials. Ionizing radiation (IR) is widely used as a potent genotoxic agent in plant DDR research as well as plant breeding and quarantine services for commercial uses. This review aimed to highlight the recent advances in cellular and phenotypic DDRs, especially those induced by IR. Various physicochemical genotoxic agents damage DNA directly or indirectly by inhi...
3 CitationsSource
#1Takeshi Hirakawa (Ridai: Tokyo University of Science)H-Index: 5
#2Keiko Kuwata (Nagoya University)H-Index: 14
Last. Sachihiro Matsunaga (Ridai: Tokyo University of Science)H-Index: 38
view all 7 authors...
Homologous recombination is a key process for maintaining genome integrity and diversity. In eukaryotes, the nucleosome structure of chromatin inhibits the progression of homologous recombination. The DNA repair and recombination protein RAD54 alters the chromatin structure via nucleosome sliding to enable homology searches. For homologous recombination to progress, appropriate recruitment and dissociation of RAD54 is required at the site of homologous recombination; however, little is known abo...
2 CitationsSource
#1Jianbin Lai (SCNU: South China Normal University)H-Index: 10
#2Jieming Jiang (SCNU: South China Normal University)H-Index: 4
Last. Chengwei Yang (SCNU: South China Normal University)H-Index: 18
view all 7 authors...
DNA damage occurs in all cells and can hinder chromosome stability and cell viability. Structural Maintenance of Chromosomes 5/6 (SMC5/6) is a protein complex that functions as an evolutionarily conserved chromosomal ATPase critical for repairing DNA double-strand breaks (DSBs). However, the mechanisms regulating this complex in plants are poorly understood. Here, we identified the transcriptional coactivator ALTERATION/DEFICIENCY IN ACTIVATION 2B (ADA2b) as an interactor of SMC5 in Arabidopsis ...
1 CitationsSource