In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni.

Volume: 8, Issue: 1, Pages: 14500 - 14500
Published: Feb 21, 2017
Abstract
Several CRISPR-Cas9 orthologues have been used for genome editing. Here, we present the smallest Cas9 orthologue characterized to date, derived from Campylobacter jejuni (CjCas9), for efficient genome editing in vivo. After determining protospacer-adjacent motif (PAM) sequences and optimizing single-guide RNA (sgRNA) length, we package the CjCas9 gene, its sgRNA sequence, and a marker gene in an all-in-one adeno-associated virus (AAV) vector and...
Paper Details
Title
In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni.
Published Date
Feb 21, 2017
Volume
8
Issue
1
Pages
14500 - 14500
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