Analytical Biases Associated with GC-Content in Molecular Evolution

Molecular evolution is being revolutionised by high-throughput sequencing allowing an increased amount of genome-wide data available for multiple species. While base composition summarised by GC-content is one of the first metrics measured in genomes, its genomic distribution is a frequently neglected feature in downstream analyses based on DNA sequence comparisons. Here, we show how base composition heterogeneity among loci and taxa can bias...