Optimized Enrichment of Phosphoproteomes by Fe-IMAC Column Chromatography
Abstract
Phosphorylation is among the most important post-translational modifications of proteins and has numerous regulatory functions across all domains of life. However, phosphorylation is often substoichiometric, requiring selective and sensitive methods to enrich phosphorylated peptides from complex cellular digests. Various methods have been devised for this purpose and we have recently described a Fe-IMAC HPLC column chromatography setup which is...
Paper Details
Title
Optimized Enrichment of Phosphoproteomes by Fe-IMAC Column Chromatography
Published Date
Jan 1, 2017
Journal
Pages
47 - 60
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