Manual curation and reannotation of the genomes of Clostridium difficile 630Δerm and C. difficile 630

Purpose. We resequenced the genome of Clostridium difficile 630Δerm (DSM 28645), a model strain commonly used for the generation of insertion mutants. Methodology. The genome sequence was obtained by a combination of single-molecule real-timeand Illumina sequencing technology. Results. Detailed manual curation and comparison to the previously published genomic sequence revealed sequence differences including inverted regions and the presence of...