Impact of light intensity and quality on chromatophore and nuclear gene expression in Paulinella chromatophora, an amoeba with nascent photosynthetic organelles

Published on Apr 1, 2017in Plant Journal 5.78
· DOI :10.1111/tpj.13488
Ru Zhang7
Estimated H-index: 7
(Carnegie Institution for Science),
Eva C.M. Nowack12
Estimated H-index: 12
(University of Düsseldorf)
+ 2 AuthorsArthur R. Grossman80
Estimated H-index: 80
(Carnegie Institution for Science)
Abstract
Summary Plastid evolution has been attributed to a single primary endosymbiotic event that occurred about 1.6 billion years ago (BYA) in which a cyanobacterium was engulfed and retained by a eukaryotic cell, although early steps in plastid integration are poorly understood. The photosynthetic amoeba Paulinella chromatophora represents a unique model for the study of plastid evolution because it contains cyanobacterium-derived photosynthetic organelles termed ‘chromatophores’ that originated relatively recently (0.09–0.14 BYA). The chromatophore genome is about a third the size of the genome of closely related cyanobacteria, but 10-fold larger than most plastid genomes. Several genes have been transferred from the chromatophore genome to the host nuclear genome through endosymbiotic gene transfer (EGT). Some EGT-derived proteins could be imported into chromatophores for function. Two photosynthesis-related genes (psaI and csos4A) are encoded by both the nuclear and chromatophore genomes, suggesting that EGT in Paulinella chromatophora is ongoing. Many EGT-derived genes encode proteins that function in photosynthesis and photoprotection, including an expanded family of high-light-inducible (ncHLI) proteins. Cyanobacterial hli genes are high-light induced and required for cell viability under excess light. We examined the impact of light on Paulinella chromatophora and found that this organism is light sensitive and lacks light-induced transcriptional regulation of chromatophore genes and most EGT-derived nuclear genes. However, several ncHLI genes have reestablished light-dependent regulation, which appears analogous to what is observed in cyanobacteria. We postulate that expansion of the ncHLI gene family and its regulation may reflect the light/oxidative stress experienced by Paulinella chromatophora as a consequence of the as yet incomplete integration of host and chromatophore metabolisms.
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References70
Published on Dec 1, 2015in Plant Cell Reports 2.99
Xiaofei Zeng3
Estimated H-index: 3
(Wuhan University),
Neng Cheng2
Estimated H-index: 2
(Wuhan University)
+ 5 AuthorsZhongli Hu6
Estimated H-index: 6
(Wuhan University)
Key message Six MnSOD genes were isolated from five Miscanthus species. MgMnSOD1 functions in mitochondria and MgMnSOD1 seems to be the main MnSOD gene involved in stress response of M. × giganteus.
3 Citations Source Cite
Published on Jan 1, 2002in Plant Molecular Biology 3.54
Hong Xu5
Estimated H-index: 5
(Arizona State University),
Dmitrii V. Vavilin15
Estimated H-index: 15
(Arizona State University)
+ 1 AuthorsWim F. J. Vermaas36
Estimated H-index: 36
(Arizona State University)
In the cyanobacterium Synechocystis sp. PCC 6803 five open reading frames (scpA–scpE) have been identified that code for single-helix proteins resembling helices I and III of chlorophyll a/b-binding (Cab) antenna proteins from higher plants. They have been named SCPs (small Cab-like proteins). Deletion of a single scp gene in a wild-type or in a photosystem I-less (PS I-less) strain has little effect. However, the effects of functional deletion of scpB or scpE were remarkable under conditions wh...
72 Citations Source Cite
Published on Apr 1, 2012in Plant Cell and Environment 5.42
Rakesh Kumar Sinha6
Estimated H-index: 6
,
Josef Komenda34
Estimated H-index: 34
(Sewanee: The University of the South)
+ 2 AuthorsPavel Pospíšil21
Estimated H-index: 21
The cyanobacterial small CAB-like proteins (SCPs) are single-helix membrane proteins mostly associated with the photosystem II (PSII) complex that accumulate under stress conditions. Their function is still ambiguous although they are assumed to regulate chlorophyll (Chl) biosynthesis and/or to protect PSII against oxidative damage. In this study, the effect of SCPs on the PSII-specific light-induced damage and generation of singlet oxygen (1O2) was assessed in the strains of the cyanobacterium ...
31 Citations Source Cite
Published on Jan 1, 1975in Biochemie und Physiologie der Pflanzen
S.W. Jeffrey1
Estimated H-index: 1
(University of Sydney),
G.F. Humphrey1
Estimated H-index: 1
(University of Sydney)
Summary New equations are presented for spectrophotometric determination of chlorophylls, based on revised extinction coefficients of chlorophylls a , b , c 1 and c 2 . These equations may be used for determining chlorophylls a and b in higher plants and green algae, chlorophylls a and c 1 + c 2 in brown algae, diatoms and chrysomonads, chlorophylls a and c 2 in dinoflagellates and cryptomonads, and chlorophylls a , b , and c 1 + c 2 in natural phytoplankton.
2,944 Citations Source Cite
Published on Jan 1, 2000in Plant Molecular Biology 3.54
Stefan Jansson61
Estimated H-index: 61
(Umeå University),
Jenny Andersson8
Estimated H-index: 8
(Umeå University)
+ 1 AuthorsGrzegorz Jackowski12
Estimated H-index: 12
(Umeå University)
An Arabidopsis thaliana cDNA clone encoding a novel 110 amino acid thylakoid protein has been sequenced. The in vitro synthesized protein is taken up by intact chloroplasts, inserted into the thylakoid membrane and the transit peptide is cleaved off during this process. The mature protein is predicted to contain 69 amino acids, to form one membrane-spanning α-helix and to have its N-terminus at the stromal side of the thylakoid membrane. The protein showed similarity to the LHC, ELIP and PsbS pr...
92 Citations Source Cite
Published on Dec 1, 2010in BMC Evolutionary Biology 3.03
Johannes Engelken10
Estimated H-index: 10
(Pompeu Fabra University),
Henner Brinkmann49
Estimated H-index: 49
(Université de Montréal),
Iwona Adamska18
Estimated H-index: 18
(University of Konstanz)
Background: The extended light-harvesting complex (LHC) protein superfamily is a centerpiece of eukaryotic photosynthesis, comprising the LHC family and several families involved in photoprotection, like the LHC-like and the photosystem II subunit S (PSBS). The evolution of this complex superfamily has long remained elusive, partially due to previously missing families. Results: In this study we present a meticulous search for LHC-like sequences in public genome and expressed sequence tag databa...
72 Citations Source Cite
Published on Aug 25, 2015in Investigative Ophthalmology & Visual Science 3.39
Jin Hyoung Park5
Estimated H-index: 5
(University of Ulsan),
Soon-Suk Kang6
Estimated H-index: 6
(University of Ulsan)
+ 1 AuthorsHungwon Tchah20
Estimated H-index: 20
(University of Ulsan)
PURPOSE. We evaluated the effects of N-acetylcysteine (NAC), which is known to inhibit reactive oxygen species (ROS)‐dependent apoptosis, on high glucose‐induced ROS, apoptosis, inflammation, and delayed-wounding closure in primary cultured human conjunctival epithelial cells (pHCECs), and the regulatory effects of cleaved caspase-3, BAX, nuclear factor jB (NF-jB), IL-6, and TNF-a on these processes. METHODS. High glucose‐induced ROS generation was measured using 2 0 ,7 0 -dichlorofluorescein di...
6 Citations Source Cite
Published on Dec 28, 2007in Journal of Biological Chemistry 4.01
Dmitrii V. Vavilin15
Estimated H-index: 15
(Arizona State University),
Danny Yao4
Estimated H-index: 4
(Arizona State University),
Willem Vermaas24
Estimated H-index: 24
Abstract Isotope (Na15NO3, (15NH4)SO4 or [13C]glucose) labeling was used to analyze chlorophyll synthesis and degradation rates in a set of Synechocystis mutants that lacked single or multiple small Cab-like proteins (SCPs), as well as photosystem I or II. When all five small Cab-like proteins were inactivated in the wild-type background, chlorophyll stability was not affected unless the scpABCDE- strain was grown at a moderately high light intensity of 100–300 μmol photons m-2 s-1. However, the...
47 Citations Source Cite
Published on Nov 1, 2000in Gene 2.50
Marie-Hélène Montané6
Estimated H-index: 6
,
Klaus Kloppstech32
Estimated H-index: 32
Abstract Light-harvesting complex proteins (LHCs) and early light-induced proteins (ELIPs) are essential pigment-binding components of the thylakoid membrane and are encoded by one of the largest and most complex higher plant gene families. The functional diversification of these proteins corresponded to the transition from extrinsic (phycobilisome-based) to intrinsic (LHC-based) light-harvesting antenna systems during the evolution of chloroplasts from cyanobacteria, yet the functional basis of...
150 Citations Source Cite
Published on Jan 5, 2001in Journal of Biological Chemistry 4.01
Qingfang He13
Estimated H-index: 13
(Carnegie Institution for Science),
Nadia Dolganov9
Estimated H-index: 9
(Carnegie Institution for Science)
+ 1 AuthorsArthur R. Grossman80
Estimated H-index: 80
(Carnegie Institution for Science)
Abstract There are five Synechocystis PCC6803 genes encoding polypeptides with similarity to the Lhc polypeptides of plants. Four of the polypeptides, designated HliA–D (Dolganov, N. A. M., Bhaya, D., and Grossman, A. R. (1995)Proc. Natl. Acad. Sci. U. S. A. 92, 636–640) (corresponding to ScpC, ScpD, ScpB, and ScpE in Funk, C., and Vermaas, W. (1999) Biochemistry 38, 9397–9404) contain a single transmembrane domain. The fifth polypeptide (HemH) represents a fusion between a ferrochelatase and an...
171 Citations Source Cite
Cited By2
Published on Jun 1, 2017in Current Opinion in Plant Biology 7.35
Guillaume Allorent6
Estimated H-index: 6
(Commissariat à l'énergie atomique et aux énergies alternatives),
Dimitris Petroutsos16
Estimated H-index: 16
(Commissariat à l'énergie atomique et aux énergies alternatives)
In photosynthetic organisms, proteins in the light-harvesting complex (LHC) harvest light energy to fuel photosynthesis, whereas photoreceptor proteins are activated by the different wavelengths of the light spectrum to regulate cellular functions. Under conditions of excess light, blue-light photoreceptors activate chloroplast avoidance movements in sessile plants, and blue- and green-light photoreceptors cause motile algae to swim away from intense light. Simultaneously, LHCs switch from light...
12 Citations Source Cite
Published on Sep 1, 2017in Current Biology 9.25
Anna Singer3
Estimated H-index: 3
(University of Düsseldorf),
Gereon Poschmann16
Estimated H-index: 16
(University of Düsseldorf)
+ 6 AuthorsEva C.M. Nowack12
Estimated H-index: 12
(University of Düsseldorf)
Summary The endosymbiotic acquisition of mitochondria and plastids more than 1 Ga ago profoundly impacted eukaryote evolution. At the heart of understanding organelle evolution is the re-arrangement of the endosymbiont proteome into a host-controlled organellar proteome. However, early stages in this process as well as the timing of events that underlie organelle integration remain poorly understood. The amoeba Paulinella chromatophora contains cyanobacterium-derived photosynthetic organelles, t...
16 Citations Source Cite