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Tissue Fixation and Processing for the Histological Identification of Lipids

Published on Jan 1, 2017in Methods of Molecular Biology
· DOI :10.1007/978-1-4939-6788-9_14
Víctor Carriel17
Estimated H-index: 17
(UGR: University of Granada),
Fernando Campos7
Estimated H-index: 7
(UGR: University of Granada)
+ 1 AuthorsJohn A. Kiernan36
Estimated H-index: 36
(UWO: University of Western Ontario)
Abstract
Lipids are a heterogeneous group of substances characterized by their solubility in organic solvents and insolubility in water. Lipids can be found as normal components of different tissues and organs, and they can be affected by several pathological conditions. The histochemical identification of lipids plays an important role in histopathological diagnosis and research, but successful staining depends on adequate fixation and processing of the tissue. Here we describe methods to fix and process tissue samples for the histochemical identification of lipids in frozen or paraffin-embedded tissues.
  • References (11)
  • Citations (7)
References11
Newest
#1Víctor Carriel (UGR: University of Granada)H-Index: 17
#2Antonio Campos (UGR: University of Granada)H-Index: 22
Last.Stefano Geuna (UNITO: University of Turin)H-Index: 44
view all 5 authors...
11 CitationsSource
12 CitationsSource
#1Víctor Carriel (UGR: University of Granada)H-Index: 17
#2Ingrid Garzón (UGR: University of Granada)H-Index: 18
Last.Maria Cornelissen (UGent: Ghent University)H-Index: 30
view all 4 authors...
18 CitationsSource
#1Víctor Carriel (UGR: University of Granada)H-Index: 17
#2Ingrid Garzón (UGR: University of Granada)H-Index: 18
Last.Antonio Campos (UGR: University of Granada)H-Index: 8
view all 4 authors...
27 CitationsSource
5 Citations
#1Federica Di Scipio (UNITO: University of Turin)H-Index: 11
#2Stefania Raimondo (UNITO: University of Turin)H-Index: 31
Last.Stefano Geuna (UNITO: University of Turin)H-Index: 44
view all 4 authors...
90 CitationsSource
25 CitationsSource
65 CitationsSource
#1Richard E. Tracy (LSU: Louisiana State University)H-Index: 21
#2Parvene Walia (LSU: Louisiana State University)H-Index: 1
7 CitationsSource
#1R E Tracy (LSU Health Sciences Center New Orleans)H-Index: 1
#2P Walia (LSU Health Sciences Center New Orleans)H-Index: 1
20 CitationsSource
Cited By7
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#1Jesús Chato-Astrain (UGR: University of Granada)H-Index: 1
#2Fernando Campos (UGR: University of Granada)H-Index: 7
Last.Víctor Carriel (UGR: University of Granada)H-Index: 17
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1 CitationsSource
#1Charlot Philips (UGent: Ghent University)H-Index: 4
#2Fernando Campos (UGR: University of Granada)H-Index: 7
Last.Víctor Carriel (UGR: University of Granada)H-Index: 17
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4 CitationsSource
#1Carlos Godoy-Guzman (Usach: University of Santiago, Chile)H-Index: 2
#2Claudio NuñezH-Index: 1
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#1Ying Qiang Shen (UGR: University of Granada)H-Index: 2
#2Ana Guerra-Librero (UGR: University of Granada)H-Index: 7
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34 CitationsSource
#1Charlot Philips (UGent: Ghent University)H-Index: 4
#2Maria Cornelissen (UGent: Ghent University)H-Index: 30
Last.Víctor Carriel (UGent: Ghent University)H-Index: 17
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7 CitationsSource
#1Francisco José Bermúdez-Jiménez (UGR: University of Granada)H-Index: 3
#2Víctor Carriel (UGR: University of Granada)H-Index: 17
Last.Juan Jiménez-Jáimez (UGR: University of Granada)H-Index: 9
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#1Víctor Carriel (UGR: University of Granada)H-Index: 17
#2Antonio Campos (UGR: University of Granada)H-Index: 22
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11 CitationsSource