Acquisition of accurate data from intramolecular quenched fluorescence protease assays
Abstract
The Intramolecular Quenched Fluorescence (IQF) protease assay utilizes peptide substrates containing donor-quencher pairs that flank the scissile bond. Following protease cleavage, the dequenched donor emission of the product is subsequently measured. Inspection of the IQF literature indicates that rigorous treatment of systematic errors in observed fluorescence arising from inner-filter absorbance (IF) and non-specific intermolecular quenching...
Paper Details
Title
Acquisition of accurate data from intramolecular quenched fluorescence protease assays
Published Date
Apr 1, 2017
Journal
Volume
522
Pages
30 - 36
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