Angiotensin-converting enzyme inhibitors attenuate propofol-induced pro-oxidative and antifibrinolytic effect in human endothelial cells.

Published on Jan 1, 2017in Journal of the Renin-Angiotensin-Aldosterone System1.356
· DOI :10.1177/1470320316687197
Marzena Wojewodzka-Zelezniakowicz6
Estimated H-index: 6
(UMB: Medical University of Białystok),
Anna Gromotowicz-Poplawska4
Estimated H-index: 4
(UMB: Medical University of Białystok)
+ 4 AuthorsEwa Chabielska16
Estimated H-index: 16
(UMB: Medical University of Białystok)
Introduction:The aim of this study was to investigate the effects of plasma and tissue angiotensin-converting enzyme inhibitors (ACE-Is) against propofol-induced endothelial dysfunction and to elucidate the involved mechanisms in vitro.Materials and methods:We examined the effects of propofol (50 μM), quinaprilat and enalaprilat (10−5 M) on fibrinolysis (t-PA, PAI-1, TAFI antigen levels), oxidative stress parameters (H2O2 and MDA antigen levels and SOD and NADPH oxidase mRNA levels) and nitric oxide bioavailability (NO2/NO3 concentration and NOS expression at the level of mRNA) in human umbilical vein endothelial cells (HUVECs).Results:We found that both ACE-Is promoted similar endothelial fibrinolytic properties and decreased oxidative stress in vitro. Propofol alone increased the release of antifibrinolytic and pro-oxidative factors from the endothelium and increased mRNA iNOS expression. We also found that the incubation of HUVECs in the presence of propofol following ACE-Is pre-incubation caused weakn...
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