Epithelial expression and function of trypsin-3 in irritable bowel syndrome

Proteases are key mediators of pain and altered enteric neuronal signalling, although the types and sources of these important intestinal mediators are unknown. We hypothesised that intestinal epithelium is a major source of trypsin-like activity in patients with IBS and this activity signals to primary afferent and enteric nerves and induces visceral hypersensitivity.Trypsin-like activity was determined in tissues from patients with IBS and in...