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Evaluation of in vitro antioxidant and anticancer activity of Allophylus cobbe leaf extracts on DU-145 and PC-3 human prostate cancer cell lines

Published on Feb 1, 2017in Cytotechnology1.67
· DOI :10.1007/s10616-016-0048-1
Shridhar C Ghagane1
Estimated H-index: 1
(Karnatak University),
Shridevi I. Puranik (Karnatak University)+ 1 AuthorsMurigendra B. Hiremath8
Estimated H-index: 8
(Karnatak University)
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Abstract
Allophylus cobbe (L.) Raeusch. belonging to the family Sapindaceae, is a commonly distributed small shrub in Western Ghats of India previously reported for its traditional medicinal properties. It is used for the treatment of various ailments. The present study is aimed at investigating preliminary phytochemicals, inducing the determination of the total phenolic contents, antioxidant assays and anticancer activity of A. cobbe leaf extracts on (DU-145) and (PC-3) cell lines. Preliminary phytochemical screening showed a broad spectrum of secondary metabolites. Highest amount of phenolic content was present in aqueous extract (91.96 ± 0.61 mg/g GAE) and it also proved to have the most potent antioxidant activity at a concentration of 100 mg/ml (64.71 ± 0.15%). IC50 value was (431.10 ± 15.05 µg/mL) for DU-145 and (362.08 ± 24.17 µg/mL) for PC-3 cell lines while the standard drug paclitaxel showed an IC50 value of 0.3 µM/mL. Morphological changes was observed in cancerous cells undergoing apoptosis in human prostate cancer cell lines (DU-145 and PC-3) while the extract showed no cytotoxicity towards normal cells (MEF-L929). It can be concluded that the tested extracts holds significant antioxidant and anticancer activities. However further investigation on lead compounds of A. cobbe will enable its therapeutic use.
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References25
Newest
Published on Mar 1, 2016
Gireesh M. Ankad3
Estimated H-index: 3
(ICMR: Indian Council of Medical Research),
Vinayak Upadhya7
Estimated H-index: 7
(ICMR: Indian Council of Medical Research)
+ 3 AuthorsSanjiva D. Kholkute10
Estimated H-index: 10
(ICMR: Indian Council of Medical Research)
Allophylus cobbe is a small tree and an ethnomedicinally important plant species belonging to the family Sapindaceae, traditionally used to treat various ailments. Previous work on the species led to show ethanolic extracts to be active against Ranikhet disease virus and have showed effects on central nervous system and hypothermia. However, literature on its polyphenolic content, antioxidant and antimicrobial activity is inadequate. Because of its wide use in traditional medicine, it was decide...
Published on Jan 1, 2016in Indian Journal of Urology
Krishnamoorthy Hariharan1
Estimated H-index: 1
,
Venugopal Padmanabha1
Estimated H-index: 1
Introduction: The incidence of prostate cancer has shown significant variation across the globe. Though the prevalence and characteristics of this disease have been extensively studied in many countries, data regarding the true incidence of prostate cancer in India is limited. Materials and Methods: MEDLINE publications from 1990 to 2014 were searched and reviewed and compiled to assess the demographic profile of prostate cancer in India and characteristics unique to this disease in India. Resul...
Published on Jan 1, 2016in Indian Journal of Urology
Krishnamoorthy Hariharan1
Estimated H-index: 1
,
Biju S Pillai1
Estimated H-index: 1
,
Devesh Bansal1
Estimated H-index: 1
Herpes zoster is a reactivated varicella-zoster virus (VZV) infection of the sensory nerve ganglion, peripheral nerve, and its branches. Mechanical trauma to the nervous system can reactivate VZV. It is well known that extracorporeal shock wave lithotripsy (SWL) can produce mechanical damage to the tissue. We report a rare case of herpes zoster reactivation after SWL for treatment of 1.2 cm size renal stone in a 63-year-old male patient.
Published on Oct 1, 2015in Cytotechnology1.67
Omayma A. Eldahshan7
Estimated H-index: 7
(Ain Shams University),
Mohamed M. Abdel-Daim22
Estimated H-index: 22
(Suez Canal University)
The strychnine tree (Strychnos nux-vomica L.) (S. nux-vomica) belonging to family Loganiaceae has been a very promising medication for certain disorders. Different chromatographic methods were used to isolate the phenolic compounds from the aqueous methanolic extract of the S. nux-vomica leaves. Their identification was achieved through spectroscopic techniques. Cytotoxicity, analgesic, antipyretic and anti-inflammatory activities of S. nux-vomica leaves extract were evaluated. Five phenolic com...
Published on Dec 1, 2014in Meta Gene
Shalu Jain1
Estimated H-index: 1
(Safdarjang Hospital),
Sunita Saxena23
Estimated H-index: 23
(Safdarjang Hospital),
Anup Kumar6
Estimated H-index: 6
(Safdarjang Hospital)
Data from national cancer registries shows that incidence of certain cancers are on rise in India. The cancers which are showing significant increase in incidence rates include prostate, mouth and kidney among male population, corpus uteri, breast and thyroid among female population and lung cancer in both male and female populations. In the present review article we have focused on epidemiology of prostate cancer in Indian subcontinent in terms of incidence, survival, and mortality etc. The art...
Published on Mar 1, 2014in Cytotechnology1.67
Nikhil Baban Ghate10
Estimated H-index: 10
(Bose Institute),
Bibhabasu Hazra13
Estimated H-index: 13
(Bose Institute)
+ 1 AuthorsNripendranath Mandal15
Estimated H-index: 15
(Bose Institute)
Spondias pinnata, a commonly distributed tree in India, previously proven for various pharmacological properties and also reported for efficient anti-oxidant, free radical scavenging and iron chelating activity, continuing this, the present study is aimed to investigate the role of 70 % methanolic extract of S. pinnata bark (SPME) in promoting apoptosis in human lung adenocarcinoma cell line (A549) and human breast adenocarcinoma cell line (MCF-7). These two malignant cell lines and a normal cel...
Published on Oct 28, 2012in journal of applied pharmaceutical science
R. Muralidharan1
Estimated H-index: 1
,
D. Narasimhan1
Estimated H-index: 1
This study is a documentation of medicinal plants used for gastro intestinal problem by villagers around Gingee hills of Villupuram District. A total of 28 Dicot plants belong to 24 families are used to cure gastrointestinal problem. Isolation of active principles and anti-microbial activity should be studied on these medicinally plants. Emphasis also made for proper documentation and conservation of these medicinal plants.
Published on Sep 1, 2012in Journal of Ethnopharmacology3.41
Pradeep Bhat3
Estimated H-index: 3
(Karnatak University),
Gurumurthi Hegde3
Estimated H-index: 3
(Karnatak University),
Ganesh R. Hegde7
Estimated H-index: 7
(Karnatak University)
Abstract Ethnopharmacological relevance Uttara Kannada district is located in the heart of the Western Ghats, one of the biodiversity hotspots, in Karnataka state of India. The thick evergreen forests are home to several ethnic communities. The study was under taken for documentation and analysis of ethnomedicinal plants in the treatment of wounds. Materials and methods Field trips were made in Uttara Kannada district to identify the key informants. The collection of information was through semi...
K. Deepti1
Estimated H-index: 1
(GITAM: Gandhi Institute of Technology and Management),
P. Umadevi1
Estimated H-index: 1
(GITAM: Gandhi Institute of Technology and Management)
+ 1 AuthorsB. Vinod polarao1
Estimated H-index: 1
(GITAM: Gandhi Institute of Technology and Management)
Abstract Objective The objective of the present work is to evaluate the presence of Phytochemical constituents and antimicrobial activity of different extracts of leaves of Morinda tinctoria Roxb. Methods The serial exhaustive extraction was done with a series of solvents: Hexane, Chloroform, Ethylacetate and Methanol with increasing polarity using soxhlet apparatus. The Phytochemical analysis was done by using the standard procedures. Antimicrobial activity was evaluated by Agar well diffusion ...
Published on Nov 1, 2010in Phytomedicine4.18
Manmeet Kumar14
Estimated H-index: 14
(Central Drug Research Institute),
Preeti Rawat16
Estimated H-index: 16
(Central Drug Research Institute)
+ 6 AuthorsRakesh Maurya32
Estimated H-index: 32
(Central Drug Research Institute)
The objective of this study was to determine the in vitro osteogenic activities of selected medicinal plants used traditionally in India. The compounds isolated from three plants viz. Allophylus serratus, Cissus quadrangularis and Vitex negundo were evaluated for their in vitro osteogenic activities. Primary cultures of osteoblasts were used to determine the effects of these components on osteoblast functions. Five (4, 6, 9, 12 and 14) of the fourteen compounds isolated led to increase in osteob...
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Published on 2019in Cell Stress & Chaperones2.90
Fatih Kar (Eskişehir Osmangazi University), Ceyhan Hacıoğlu (Düzce University)+ 2 AuthorsGüngör Kanbak12
Estimated H-index: 12
(Eskişehir Osmangazi University)
Prostate cancer is the main cause of cancer-related mortality in men around the world and an important health problem. DU-145 human prostate cancer cells provide an opportunity to investigate prostate cancer. Betaine has a number of anticancer effects, such as inactivation of carcinogens, inhibition of cancer cell proliferation, angiogenesis, and metastasis. However, there is no study investigating the effects of betaine on DU-145 cells. The aim of this study was to evaluate the effects of diffe...
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