304. Deriving Useful Data from Next-Gen Sequencing of AAV Capsid Libraries

The relatively short read lengths produced by the major Next-Gen sequencing platforms (up to 300 nt for Illumina, up to 200 nt for Ion Proton) are poorly suited for analyzing large combinatorial libraries of longer nucleotide sequences, such as those involving AAV capsid genes. Despite its much lower throughput and accuracy, the PacBio single-molecule real-time technology is currently the only option for long templates, with average read lengths...