568. Transient Manipulation of DNA Damage Repair Pathway Choice Improves Homology-Directed Repair During CRISPR/Cas9-Mediated Genome Editing

CRISPR/Cas9 system allows efficient gene ablation through error-prone non-homologous end joining DNA repair. However, very low efficiency of homology-directed DNA repair (HDR) is the bottleneck in correcting genetic mutations of clinical relevance. Here we report that transient manipulation of DNA damage repair pathways increases the HDR frequency by 3-5 fold. Furthermore, we show that this approach is applicable to introduce precise genetic...