Correlated fluorescence microscopy and cryo-electron tomography of virus-infected or transfected mammalian cells
Abstract
Correlative light and electron microscopy (CLEM) combines spatiotemporal information from fluorescence light microscopy (fLM) with high-resolution structural data from cryo-electron tomography (cryo-ET). These technologies provide opportunities to bridge knowledge gaps between cell and structural biology. Here we describe our protocol for correlated cryo-fLM, cryo-electron microscopy (cryo-EM), and cryo-ET (i.e., cryo-CLEM) of virus-infected or...
Paper Details
Title
Correlated fluorescence microscopy and cryo-electron tomography of virus-infected or transfected mammalian cells
Published Date
Dec 15, 2016
Journal
Volume
12
Issue
1
Pages
150 - 167
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