Plasma membrane ATPase and H+ transport activities of microsomal membranes from wheat roots under Ni deficiency conditions as affected by exogenous histidine

ATPase activity and ATP-dependent H+ transport have been analyzed in membrane vesicles isolated from a winter wheat cultivar (Triticum aestivum cv. Back Cross Rushan) which was exposed to different concentrations of nickel (Ni) and histidine (His). Seedlings were grown in a modified Johnson nutrient solution and then exposed to the uptake solution containing 10 μM Ni to which either 100 μM His or no amino acid was added. Control plants were...