Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array

Volume: 35, Issue: 1, Pages: 31 - 34
Published: Jan 1, 2017
Abstract
Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain,...
Paper Details
Title
Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array
Published Date
Jan 1, 2017
Volume
35
Issue
1
Pages
31 - 34
Citation AnalysisPro
  • Scinapse’s Top 10 Citation Journals & Affiliations graph reveals the quality and authenticity of citations received by a paper.
  • Discover whether citations have been inflated due to self-citations, or if citations include institutional bias.