Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ
Abstract
Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration into the target chromosome (PITCh) system for a gene cassette knock-in without long homology arms mediated by microhomology-mediated end-joining. Here, we identified exonuclease 1 (Exo1) as an enhancer for PITCh...
Paper Details
Title
Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ
Published Date
Nov 28, 2016
Journal
Volume
17
Issue
1
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