Deciphering characteristics of the designer cellulosome from Bacillus subtilis WB800N via enzymatic analysis
Abstract
Recombinant Bacillus subtilis WB800N harboring pGETS 118 designer cellulosome genes was applied to the artificial cellulosome production. To investigate the characteristics of cellulosome assembly in the supernatant, three free enzymes clones, i.e., endoglucanase, exoglucanase and xylanase, were constructed. The samples of the respective free enzymes and the cellulosome were collected and examined via various enzymatic assays. In comparison of...
Paper Details
Title
Deciphering characteristics of the designer cellulosome from Bacillus subtilis WB800N via enzymatic analysis
Published Date
Jan 1, 2017
Volume
117
Pages
147 - 155
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