Dynamic Regulation of a Ribosome Rescue Pathway in Erythroid Cells and Platelets

Protein synthesis continues in platelets and maturing reticulocytes, although these blood cells lack nuclei and do not make new mRNA or ribosomes. Here, we analyze translation in primary human cells from anucleate lineages by ribosome profiling and uncover a dramatic accumulation of post-termination unrecycled ribosomes in the 3′ UTRs of mRNAs. We demonstrate that these ribosomes accumulate as a result of the natural loss of the ribosome...