Biosynthesis of poly(2-hydroxyisovalerate-co-lactate) by metabolically engineeredEscherichia coli

Polyhydroxyalkanoates (PHAs) containing 2‐hydroxyacids such as lactate (LA) and 2‐hydroxybutyrate (2HB) have recently been produced by metabolically engineered microorganisms. Here, we further expanded 2‐hydroxyacid monomer spectrum of PHAs by engineering Escherichia coli to produce PHAs containing 2‐hydroxyisovalerate (2HIV). To generate 2HIV in vivo, feedback resistant ilvBN mut genes encoding acetohydroxyacid synthase and ilvCD genes encoding...