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  • Citations (43)
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References25
Newest
Published on Nov 1, 2016in Scientific Reports 4.12
Kwangryul Baek5
Estimated H-index: 5
(Hanyang University),
Duk Hyoung Kim6
Estimated H-index: 6
(Hanyang University)
+ 5 AuthorsSangsu Bae13
Estimated H-index: 13
(Hanyang University)
Microalgae are versatile organisms capable of converting CO2, H2O, and sunlight into fuel and chemicals for domestic and industrial consumption. Thus, genetic modifications of microalgae for enhancing photosynthetic productivity, and biomass and bio-products generation are crucial for both academic and industrial applications. However, targeted mutagenesis in microalgae with CRISPR-Cas9 is limited. Here we report, a one-step transformation of Chlamydomonas reinhardtii by the DNA-free CRISPR-Cas9...
72 Citations Source Cite
Published on Aug 1, 2016in Nature Biotechnology 35.72
Y.-G. Kim8
Estimated H-index: 8
,
Seung-A. Cheong1
Estimated H-index: 1
+ 4 AuthorsYoung Hoon Sung5
Estimated H-index: 5
64 Citations Source Cite
Published on Aug 1, 2016in Nature Biotechnology 35.72
Daesik Kim12
Estimated H-index: 12
,
Jeong Eun Kim15
Estimated H-index: 15
+ 3 AuthorsJin-Soo Kim52
Estimated H-index: 52
On-target and off-target specificities of Cfp1 are assessed and compared with SpCas9 in human cells.
200 Citations Source Cite
Published on Aug 1, 2016in Nature Biotechnology 35.72
Junho K. Hur3
Estimated H-index: 3
,
Kyoungmi Kim7
Estimated H-index: 7
+ 6 AuthorsJinsoo Kim49
Estimated H-index: 49
82 Citations Source Cite
Published on Jul 1, 2016in Nature Biotechnology 35.72
Luca Pinello20
Estimated H-index: 20
,
Matthew C. Canver13
Estimated H-index: 13
+ 4 AuthorsGuo-Cheng Yuan39
Estimated H-index: 39
116 Citations Source Cite
Published on Jan 22, 2016in Science 41.06
Christopher E. Nelson18
Estimated H-index: 18
(Duke University),
Chady H. Hakim15
Estimated H-index: 15
(MU: University of Missouri)
+ 11 AuthorsWinston X. Yan15
Estimated H-index: 15
(MIT: Massachusetts Institute of Technology)
Duchenne muscular dystrophy (DMD) is a devastating disease affecting about 1 out of 5000 male births and caused by mutations in the dystrophin gene. Genome editing has the potential to restore expression of a modified dystrophin gene from the native locus to modulate disease progression. In this study, adeno-associated virus was used to deliver the CRISPR/Cas9 system to the mdx mouse model of DMD to remove the mutated exon 23 from the dystrophin gene. This includes local and systemic delivery to...
461 Citations Source Cite
Published on Jan 1, 2016in Nature 41.58
Benjamin P. Kleinstiver12
Estimated H-index: 12
(Harvard University),
Vikram Pattanayak11
Estimated H-index: 11
(Harvard University)
+ 4 AuthorsJ. Keith Joung60
Estimated H-index: 60
(Harvard University)
A high-fidelity variant of Streptococcus pyogenes CRISPR–Cas9 is reported that lacks detectable off-target events as assessed by genome-wide break capture and targeted sequencing methods.
797 Citations Source Cite
Published on Oct 1, 2015in Cell 31.40
Bernd Zetsche11
Estimated H-index: 11
,
Jonathan S. Gootenberg19
Estimated H-index: 19
+ 9 AuthorsAviv Regev97
Estimated H-index: 97
(MIT: Massachusetts Institute of Technology)
The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi-protein complexes, whereas class 2 effectors rely on single-component effector proteins such as the well-characterized Cas9. Here, we report characterization of Cpf1, a putative class 2 CRISPR effector. We demonstrate that Cpf1 mediates robust DNA interference with features distinct from Cas9. Cpf1 is a single RNA-guid...
1,090 Citations Source Cite
Published on Sep 1, 2015in Molecular Plant 9.33
Liang-Jiao Xue10
Estimated H-index: 10
(UGA: University of Georgia),
Chung-Jui Tsai27
Estimated H-index: 27
(UGA: University of Georgia)
Knockout experiments are critical for the evaluation of gene function. Researchers have increasingly relied on genome editing technologies for precise mutagenesis at loci of interest, using engineered nucleases such as Zinc finger nucleases, transcription activator-like effector nucleases (TALENs), and CRISPR (clustered regularly interspaced short palindromic repeats)-associated proteins. Sequence-specific targeting and cleavage by these systems generate double-stranded breaks and trigger endoge...
9 Citations Source Cite
Published on May 1, 2015in Scientific Reports 4.12
Xiaoxiao Zhu7
Estimated H-index: 7
,
Yajie Xu1
Estimated H-index: 1
+ 11 AuthorsDongdong Fan3
Estimated H-index: 3
The rapid generation of various species and strains of laboratory animals using CRISPR/Cas9 technology has dramatically accelerated the interrogation of gene function in vivo. So far, the dominant approach for genotyping of genome-modified animals has been the T7E1 endonuclease cleavage assay. Here, we present a polyacrylamide gel electrophoresis-based (PAGE) method to genotype mice harboring different types of indel mutations. We developed 6 strains of genome-modified mice using CRISPR/Cas9 sys...
92 Citations Source Cite
Cited By43
Newest
Published on Mar 20, 2019in Scientific Reports 4.12
You Kyeong Jeong1
Estimated H-index: 1
(Hanyang University),
Jihyeon Yu7
Estimated H-index: 7
(Hanyang University),
Sangsu Bae13
Estimated H-index: 13
(Hanyang University)
Molecular cloning is an essential technique in molecular biology and biochemistry, but it is frequently laborious when adequate restriction enzyme recognition sites are absent. Cas9 endonucleases can induce site-specific DNA double-strand breaks at sites homologous to their guide RNAs, rendering an alternative to restriction enzymes. Here, by combining DNA cleavage via a Cas9 endonuclease and DNA ligation via Gibson assembly, we demonstrate a precise and practical DNA cloning method for replacin...
1 Citations Source Cite
Published on Mar 12, 2019in Scientific Reports 4.12
Jon P. Connelly1
Estimated H-index: 1
(St. Jude Children's Research Hospital),
Shondra M. Pruett-Miller6
Estimated H-index: 6
(St. Jude Children's Research Hospital)
CRISPR-Cas9 technology allows the creation of user-defined genomic modifications in cells and whole organisms. However, quantifying editing rates in pools of cells or identifying correctly edited clones is tedious. Targeted next-generation sequencing provides a high-throughput platform for optimizing editing reagents and identifying correctly modified clones, but the large amount of data produced can be difficult to analyze. Here, we present CRIS.py, a simple and highly versatile python-based pr...
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Published on May 13, 2019in International Journal of Molecular Sciences 3.69
Amiram Ravid25
Estimated H-index: 25
,
Noa Rapaport + 4 AuthorsRomy Zemel15
Estimated H-index: 15
Previously, we have reported that the active vitamin D metabolite, calcitriol and vitamin D3 (cholecalciferol), both remarkably inhibit hepatitis C virus production. The mechanism by which vitamin D3 exerts its effect is puzzling due to the low levels of calcitriol produced in vitamin D3-treated Huh7.5 cells. In this study, we aimed to explore the mechanism of vitamin D3 anti-hepatitis C virus effect. We show that vitamin D3 activity is not mediated by its metabolic conversion to calcitriol, but...
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Published on May 7, 2019in Nucleic Acids Research 11.56
Baisong Lu13
Estimated H-index: 13
(Wake Forest Institute for Regenerative Medicine),
Parisa Javidi-Parsijani1
Estimated H-index: 1
(Wake Forest Institute for Regenerative Medicine)
+ 7 AuthorsAtalaAnthony101
Estimated H-index: 101
(Wake Forest Institute for Regenerative Medicine)
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Published on May 1, 2019in Plant Journal 5.78
Carla Schmidt1
Estimated H-index: 1
,
Michael Pacher8
Estimated H-index: 8
,
Holger Puchta48
Estimated H-index: 48
1 Citations Source Cite
Published on Mar 15, 2019in Journal of Immunology 4.54
Pietro Crivello6
Estimated H-index: 6
,
Müberra Ahci2
Estimated H-index: 2
+ 8 AuthorsKatharina Fleischhauer27
Estimated H-index: 27
Comprehensive knockout of HLA class II (HLA-II) β-chain genes is complicated by their high polymorphism. In this study, we developed CRISPR/Cas9 genome editing to simultaneously target HLA-DRB, -DQB1, and -DPB1 through a single guide RNA recognizing a conserved region in exon 2. Abrogation of HLA-II surface expression was achieved in five different HLA-typed, human EBV-transformed B lymphoblastoid cell lines (BLCLs). Next-generation sequencing–based detection confirmed specific genomic insertion...
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