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References25
Newest
Published on Nov 1, 2016in Scientific Reports4.01
Kwangryul Baek5
Estimated H-index: 5
(Hanyang University),
Duk Hyoung Kim6
Estimated H-index: 6
(Hanyang University)
+ 5 AuthorsSangsu Bae13
Estimated H-index: 13
(Hanyang University)
Microalgae are versatile organisms capable of converting CO2, H2O, and sunlight into fuel and chemicals for domestic and industrial consumption. Thus, genetic modifications of microalgae for enhancing photosynthetic productivity, and biomass and bio-products generation are crucial for both academic and industrial applications. However, targeted mutagenesis in microalgae with CRISPR-Cas9 is limited. Here we report, a one-step transformation of Chlamydomonas reinhardtii by the DNA-free CRISPR-Cas9...
Published on Aug 1, 2016in Nature Biotechnology31.86
Y.-G. Kim8
Estimated H-index: 8
,
Seung-A. Cheong1
Estimated H-index: 1
+ 4 AuthorsYoung Hoon Sung5
Estimated H-index: 5
Published on Aug 1, 2016in Nature Biotechnology31.86
Daesik Kim12
Estimated H-index: 12
,
Jeong Eun Kim15
Estimated H-index: 15
+ 3 AuthorsJin-Soo Kim52
Estimated H-index: 52
On-target and off-target specificities of Cfp1 are assessed and compared with SpCas9 in human cells.
Published on Aug 1, 2016in Nature Biotechnology31.86
Junho K. Hur3
Estimated H-index: 3
,
Kyoungmi Kim7
Estimated H-index: 7
+ 6 AuthorsJinsoo Kim49
Estimated H-index: 49
Published on Jul 1, 2016in Nature Biotechnology31.86
Luca Pinello20
Estimated H-index: 20
,
Matthew C. Canver13
Estimated H-index: 13
+ 4 AuthorsGuo-Cheng Yuan39
Estimated H-index: 39
Published on Jan 22, 2016in Science41.04
Christopher E. Nelson18
Estimated H-index: 18
(Duke University),
Chady H. Hakim15
Estimated H-index: 15
(MU: University of Missouri)
+ 11 AuthorsWinston X. Yan15
Estimated H-index: 15
(MIT: Massachusetts Institute of Technology)
Duchenne muscular dystrophy (DMD) is a devastating disease affecting about 1 out of 5000 male births and caused by mutations in the dystrophin gene. Genome editing has the potential to restore expression of a modified dystrophin gene from the native locus to modulate disease progression. In this study, adeno-associated virus was used to deliver the CRISPR/Cas9 system to the mdx mouse model of DMD to remove the mutated exon 23 from the dystrophin gene. This includes local and systemic delivery to...
Published on Jan 1, 2016in Nature43.07
Benjamin P. Kleinstiver14
Estimated H-index: 14
(Harvard University),
Vikram Pattanayak11
Estimated H-index: 11
(Harvard University)
+ 4 AuthorsJ. Keith Joung60
Estimated H-index: 60
(Harvard University)
A high-fidelity variant of Streptococcus pyogenes CRISPR–Cas9 is reported that lacks detectable off-target events as assessed by genome-wide break capture and targeted sequencing methods.
Published on Oct 1, 2015in Cell36.22
Bernd Zetsche11
Estimated H-index: 11
,
Jonathan S. Gootenberg19
Estimated H-index: 19
+ 9 AuthorsAviv Regev98
Estimated H-index: 98
(MIT: Massachusetts Institute of Technology)
The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi-protein complexes, whereas class 2 effectors rely on single-component effector proteins such as the well-characterized Cas9. Here, we report characterization of Cpf1, a putative class 2 CRISPR effector. We demonstrate that Cpf1 mediates robust DNA interference with features distinct from Cas9. Cpf1 is a single RNA-guid...
Published on Sep 1, 2015in Molecular Plant10.81
Liang-Jiao Xue10
Estimated H-index: 10
(UGA: University of Georgia),
Chung-Jui Tsai27
Estimated H-index: 27
(UGA: University of Georgia)
Knockout experiments are critical for the evaluation of gene function. Researchers have increasingly relied on genome editing technologies for precise mutagenesis at loci of interest, using engineered nucleases such as Zinc finger nucleases, transcription activator-like effector nucleases (TALENs), and CRISPR (clustered regularly interspaced short palindromic repeats)-associated proteins. Sequence-specific targeting and cleavage by these systems generate double-stranded breaks and trigger endoge...
Published on May 1, 2015in Scientific Reports4.01
Xiaoxiao Zhu7
Estimated H-index: 7
,
Yajie Xu1
Estimated H-index: 1
+ 11 AuthorsDongdong Fan3
Estimated H-index: 3
The rapid generation of various species and strains of laboratory animals using CRISPR/Cas9 technology has dramatically accelerated the interrogation of gene function in vivo. So far, the dominant approach for genotyping of genome-modified animals has been the T7E1 endonuclease cleavage assay. Here, we present a polyacrylamide gel electrophoresis-based (PAGE) method to genotype mice harboring different types of indel mutations. We developed 6 strains of genome-modified mice using CRISPR/Cas9 sys...
Cited By43
Newest
Published on Mar 20, 2019in Scientific Reports4.01
You Kyeong Jeong1
Estimated H-index: 1
(Hanyang University),
Jihyeon Yu7
Estimated H-index: 7
(Hanyang University),
Sangsu Bae13
Estimated H-index: 13
(Hanyang University)
Molecular cloning is an essential technique in molecular biology and biochemistry, but it is frequently laborious when adequate restriction enzyme recognition sites are absent. Cas9 endonucleases can induce site-specific DNA double-strand breaks at sites homologous to their guide RNAs, rendering an alternative to restriction enzymes. Here, by combining DNA cleavage via a Cas9 endonuclease and DNA ligation via Gibson assembly, we demonstrate a precise and practical DNA cloning method for replacin...
Published on Mar 12, 2019in Scientific Reports4.01
Jon P. Connelly1
Estimated H-index: 1
(St. Jude Children's Research Hospital),
Shondra M. Pruett-Miller6
Estimated H-index: 6
(St. Jude Children's Research Hospital)
CRISPR-Cas9 technology allows the creation of user-defined genomic modifications in cells and whole organisms. However, quantifying editing rates in pools of cells or identifying correctly edited clones is tedious. Targeted next-generation sequencing provides a high-throughput platform for optimizing editing reagents and identifying correctly modified clones, but the large amount of data produced can be difficult to analyze. Here, we present CRIS.py, a simple and highly versatile python-based pr...
Published on Jul 12, 2019in Nucleic Acids Research11.15
Pin Lyu (Anhui Normal University), Parisa Javidi-Parsijani1
Estimated H-index: 1
(Wake Forest Institute for Regenerative Medicine)
+ 1 AuthorsBaisong Lu13
Estimated H-index: 13
(Wake Forest Institute for Regenerative Medicine)
Published on 2019in Plant Biotechnology Reports1.26
So Young Jeong (CNU: Chungnam National University), Hyo-Min Ahn1
Estimated H-index: 1
+ 7 AuthorsYong Pyo Lim33
Estimated H-index: 33
(CNU: Chungnam National University)
The CRISPR system enables us to induce precisely targeted mutations in a plant genome. The widely used CRISPR system is composed of a Cas9 protein derived from Streptococcus pyogenes (SpCas9) and a target site-specific guide RNA. In this study, we successfully generated the early-flowering Chinese cabbage (Brassica rapa spp. pekinensis), which is one of the most important vegetables in the world. To generate early-flowering B. rapa without requiring vernalization, we designed seven guide RNAs wh...
Published on Sep 23, 2019in Nature Biotechnology31.86
Heon Seok Kim6
Estimated H-index: 6
,
You Kyeong Jeong1
Estimated H-index: 1
(Hanyang University)
+ 2 AuthorsSangsu Bae13
Estimated H-index: 13
(Hanyang University)
Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5–7) and in a confined TC*N sequence context. Adenine base editor–induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-speci...
Published on Sep 19, 2019in bioRxiv
Kirk T Ehmsen (UCSF: University of California, San Francisco), Matthew T Knuesel (UCSF: University of California, San Francisco)+ 3 AuthorsKeith R. Yamamoto79
Estimated H-index: 79
(UCSF: University of California, San Francisco)
Background: Genetic alteration of candidate response elements at their native chromosomal loci is the only valid determinant of their potential transcriptional regulatory activities. Targeted DNA cleavage by Cas9 coupled with cellular repair processes can produce arrays of alleles that can be defined by massively parallel sequencing by synthesis (SBS), presenting an opportunity to generate and survey edited cell populations that include informative alterations. Such editing efforts commonly rely...
Published on Sep 9, 2019in Nature Nanotechnology33.41
Guojun Chen15
Estimated H-index: 15
(UW: University of Wisconsin-Madison),
Amr A. Abdeen11
Estimated H-index: 11
(UW: University of Wisconsin-Madison)
+ 7 AuthorsShaoqin Gong51
Estimated H-index: 51
Delivery technologies for the CRISPR-Cas9 (CRISPR, clustered regularly interspaced short palindromic repeats) gene editing system often require viral vectors, which pose safety concerns for therapeutic genome editing1. Alternatively, cationic liposomal components or polymers can be used to encapsulate multiple CRISPR components into large particles (typically >100 nm diameter); however, such systems are limited by variability in the loading of the cargo. Here, we report the design of customizabl...
Published on Sep 6, 2019in Plant Cell Tissue and Organ Culture2.20
Ester Stajič (University of Ljubljana), Agnieszka Kiełkowska4
Estimated H-index: 4
(H.I., S.I.: University of Agriculture, Faisalabad)
+ 1 AuthorsBorut Bohanec21
Estimated H-index: 21
(University of Ljubljana)
CRISPR/Cas9 is a versatile and highly efficient genome editing tool used in many different plant species. In the present study, we compared the two most commonly used transient expression methods for genome editing, protoplast transfection and infiltration of Agrobacterium tumefaciens, to develop a rapid and efficient validation protocol. Vectors designed to target four different sites in the cabbage genome (two of which were model target genes and two related to the centromere-specific histone ...
Published on Jun 3, 2019in bioRxiv
Hui-Min Chen (HHMI: Howard Hughes Medical Institute), Jorge García-Marqués6
Estimated H-index: 6
+ 3 AuthorsTzumin Lee27
Estimated H-index: 27
Interrogating the genome, especially locating critical cis-elements in non-protein coding regions, requires efficient deletion analysis. Conventional deletion analyses using synthetic constructs are cumbersome and nonphysiological. Here we invent CAMIO (Cas9-mediated Arrayed Mutagenesis of Individual Offspring), to achieve high-throughput organism-level structure-functional analysis of native DNA sequences. CAMIO permits creating arrays of targeted deletions directly in the genome. First, we ass...
View next paperHighly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins