Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Coconversion
Abstract
Adoption of a streamlined version of the bacterial clustered regular interspersed short palindromic repeat (CRISPR)/Cas9 defense system has accelerated targeted genome engineering. The Streptococcus pyogenes Cas9 protein, directed by a simplified, CRISPR-like single-guide RNA, catalyzes a double-stranded DNA break at a specific genomic site; subsequent repair by end joining can introduce mutagenic insertions or deletions, while repair by...
Paper Details
Title
Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Coconversion
Published Date
Oct 1, 2016
Journal
Volume
6
Issue
10
Pages
3197 - 3206
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