Kinetics of infected insect cell osmolysis and enhanced protein release using a modified disruption method

Volume: 39, Issue: 11, Pages: 1729 - 1735
Published: Jul 19, 2016
Abstract
We have studied and characterized a cell disruption method to produce a protein extract from recombinant Baculovirus infected insect cells based on osmotic lysis. Cell lysis kinetics were measured during a 24-h incubation in lysis buffer and resulting data sets were curve fitted to a hyperbola, visually similar to the Michaelis-Menten curve, to determine the maximum concentration of released protein and the time required to reach equilibrium....
Paper Details
Title
Kinetics of infected insect cell osmolysis and enhanced protein release using a modified disruption method
Published Date
Jul 19, 2016
Volume
39
Issue
11
Pages
1729 - 1735
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