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Cytochrome P450 gene, CYP4G51, modulates hydrocarbon production in the pea aphid, Acyrthosiphon pisum

Published on Sep 1, 2016in Insect Biochemistry and Molecular Biology3.62
· DOI :10.1016/j.ibmb.2016.07.006
Nan Chen3
Estimated H-index: 3
(NWAFU: Northwest A&F University),
Yongliang Fan15
Estimated H-index: 15
(NWAFU: Northwest A&F University)
+ 3 AuthorsTong-Xian Liu1
Estimated H-index: 1
(NWAFU: Northwest A&F University)
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Abstract
Terrestrial insects deposit a layer of hydrocarbons (HCs) as waterproofing agents on their epicuticle. The insect-specific CYP4G genes, subfamily members of P450, have been found in all insects with sequenced genomes to date. They are critical for HC biosynthesis in Drosophila; however, their functional roles in other insects including the piercing-sucking hemipterous aphids remain unknown. In this study, we presented the molecular characterization and a functional study of the CYP4G51 gene in the pea aphid, Acyrthosiphon pisum (Harris). CYP4G51 transcript was detectable across the whole life cycle of A. pisum, and was prominently expressed in the aphid head and abdominal cuticle. Up-regulation of CYP4G51 under desiccation stress was more significant in the third instar nymphs compared with the adults. Also, up-regulation of CYP4G51 was observed when the aphids fed on an artificial diet compared with those fed on the broad bean plant, and was positively correlated with a high level of cuticular HCs (CHCs). RNAi knockdown of CYP4G51 significantly reduced its expression and caused reductions in both internal and external HCs. A deficiency in CHCs resulted in aphids being more susceptible to desiccation, with increased mortality under desiccation stress. The current results confirm that CYP4G51 modulates HC biosynthesis to protect aphids from desiccation. Moreover, our data also indicate that saturated and straight-chain HCs play a major role in cuticular waterproofing in the pea aphid. A. pisum CYP4G51 could be considered as a novel RNAi target in the field of insect pest management.
  • References (69)
  • Citations (18)
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References69
Newest
Published on Feb 1, 2016in Insect Biochemistry and Molecular Biology3.62
Thiago A. Franco3
Estimated H-index: 3
(UFRJ: Federal University of Rio de Janeiro),
Daniele S. Oliveira2
Estimated H-index: 2
(UFRJ: Federal University of Rio de Janeiro)
+ 2 AuthorsAna C.A. Melo10
Estimated H-index: 10
(UC Davis: University of California, Davis)
Abstract Olfaction is one of the main sensory modalities that allow insects to interpret their environment. Several proteins, including odorant-binding proteins (OBPs) and odorant receptors (ORs), are involved in this process. Odorant receptors are ion channels formed by a binding unit OR and an odorant receptor co-receptor (Orco). The main goal of this study was to characterize the Orco gene of Rhodnius prolixus ( RproOrco) and to infer its biological functions using gene silencing. The full-le...
Published on Jan 1, 2016in Insect Biochemistry and Molecular Biology3.62
Senne Dillen10
Estimated H-index: 10
(Katholieke Universiteit Leuven),
Ziwei Chen1
Estimated H-index: 1
(Katholieke Universiteit Leuven),
Jozef Vanden Broeck35
Estimated H-index: 35
(Katholieke Universiteit Leuven)
Peptides of the short neuropeptide F (sNPF) family modulate feeding behavior in a wide variety of insect species, including the desert locust, Schistocerca gregaria. Likewise, the nutritional state of the animal can strongly affect sNPF expression. Although several studies have been published describing these nutrient-dependent effects, it remains largely unclear how they are achieved. In this study, we describe a series of in vivo experiments which indicate that it is not the act of feeding in ...
Published on Nov 14, 2015in eLife7.55
Lauren M. Dembeck4
Estimated H-index: 4
(NCSU: North Carolina State University),
Katalin Böröczky6
Estimated H-index: 6
(NCSU: North Carolina State University)
+ 3 AuthorsTrudy F. C. Mackay63
Estimated H-index: 63
(NCSU: North Carolina State University)
The outermost layer of an insect’s body is called the epicuticle and is made of a blend of fat molecules. “Cuticular hydrocarbons” (or CHCs) are the most common fat molecules in the epicuticle, and play an important role in protecting the insect’s body from harsh, dry habitats. CHCs also have other roles in insect behavior. For example, these molecules act as chemical cues when insects search for mates (i.e. pheromones), and they can even contribute to camouflage. Insects are amongst the most di...
Published on Nov 1, 2015in Journal of Lipid Research4.74
Claude Wicker-Thomas14
Estimated H-index: 14
(University of Paris-Sud),
Damien Garrido3
Estimated H-index: 3
(University of Paris-Sud)
+ 6 AuthorsJacques Montagne13
Estimated H-index: 13
(University of Paris-Sud)
In terrestrial insects, cuticular hydrocarbons (CHCs) provide protection from desiccation. Specific CHCs can also act as pheromones, which are important for successful mating. Oenocytes are abdominal cells thought to act as specialized units for CHC biogenesis that consists of long-chain fatty acid (LCFA) synthesis, optional desaturation(s), elongation to very long-chain fatty acids (VLCFAs), and removal of the carboxyl group. By investigating CHC biogenesis in Drosophila melanogaster, we showed...
Published on May 1, 2015in Pesticide Biochemistry and Physiology2.87
Young Ho Kim2
Estimated H-index: 2
(KSU: Kansas State University),
Moustapha Soumaila Issa1
Estimated H-index: 1
(KSU: Kansas State University)
+ 1 AuthorsKun Yan Zhu36
Estimated H-index: 36
(KSU: Kansas State University)
Abstract Since its discovery, RNA interference (RNAi) has revolutionized functional genomic studies due to its sequence-specific nature of post-transcriptional gene silencing. In this paper, we provide a comprehensive review of the recent literature and summarize the current knowledge and advances in the applications of RNAi technologies in the field of insect toxicology and insect pest management. Many recent studies have focused on identification and validation of the genes encoding insecticid...
Published on Feb 18, 2015in PLOS Genetics5.22
Damien Garrido3
Estimated H-index: 3
(University of Paris-Sud),
Thomas Rubin4
Estimated H-index: 4
(University of Paris-Sud)
+ 4 AuthorsJacques Montagne13
Estimated H-index: 13
(University of Paris-Sud)
Fatty acid (FA) metabolism is deregulated in several human diseases including metabolic syndrome, type 2 diabetes and cancers. Therefore, FA-metabolic enzymes are potential targets for drug therapy, although the consequence of these treatments must be precisely evaluated at the organismal and cellular levels. In healthy organism, synthesis of triacylglycerols (TAGs)—composed of three FA units esterified to a glycerol backbone—is increased in response to dietary sugar. Saturation in the storage a...
Published on Feb 1, 2015in Insect Biochemistry and Molecular Biology3.62
Torsten Will15
Estimated H-index: 15
(University of Giessen),
Andreas Vilcinskas38
Estimated H-index: 38
(University of Giessen)
Abstract Aphids produce two types of saliva that mediate their interactions with plants. Watery saliva is secreted during cell penetration and ingestion, whereas gel saliva is secreted during stylet movement through the apoplast where it forms a sheath around the stylet to facilitate penetration and seal puncture sites on cell membranes. In order to study the function of the sheath when aphids interact with plants, we used RNA interference (RNAi) to silence the aphid structural sheath protein (S...
Published on Aug 1, 2014in Insect Biochemistry and Molecular Biology3.62
Panagiotis Sapountzis13
Estimated H-index: 13
(University of Lyon),
Gabrielle Duport9
Estimated H-index: 9
(University of Lyon)
+ 7 AuthorsFederica Calevro15
Estimated H-index: 15
(University of Lyon)
Abstract RNA interference (RNAi) has been widely and successfully used for gene inactivation in insects, including aphids, where dsRNA administration can be performed either by feeding or microinjection. However, several aspects related to the aphid response to RNAi, as well as the influence of the administration method on tissue response, or the mixed success to observe phenotypes specific to the gene targeted, are still unclear in this insect group. In the present study, we made the first dire...
Published on Mar 7, 2014in Science41.04
Henry Chung13
Estimated H-index: 13
(HHMI: Howard Hughes Medical Institute),
David W. Loehlin2
Estimated H-index: 2
(HHMI: Howard Hughes Medical Institute)
+ 3 AuthorsSean B. Carroll77
Estimated H-index: 77
(HHMI: Howard Hughes Medical Institute)
Evolutionary changes in traits involved in both ecological divergence and mate choice may produce reproductive isolation and speciation. However, there are few examples of such dual traits, and the genetic and molecular bases of their evolution have not been identified. We show that methyl-branched cuticular hydrocarbons (mbCHCs) are a dual trait that affects both desiccation resistance and mate choice in Drosophila serrata . We identify a fatty acid synthase mFAS ( CG3524 ) responsible for mbCH...
Published on Jan 7, 2014in Annual Review of Entomology11.80
Rami Makki2
Estimated H-index: 2
,
Einat Cinnamon2
Estimated H-index: 2
,
Alex P. Gould1
Estimated H-index: 1
(Medical Research Council)
Oenocytes have intrigued insect physiologists since the nineteenth century. Many years of careful but mostly descriptive research on these cells highlights their diverse sizes, numbers, and anatomical distributions across Insecta. Contemporary molecular genetic studies in Drosophila melanogaster and Tribolium castaneum support the hypothesis that oenocytes are of ectodermal origin. They also suggest that, in both short and long germ-band species, oenocytes are induced from a Spalt major/Engraile...
Cited By18
Newest
Published on 2019in Insect Biochemistry and Molecular Biology3.62
Xiao‐Jin Pei (NWAFU: Northwest A&F University), Nan Chen3
Estimated H-index: 3
(NWAFU: Northwest A&F University)
+ 4 AuthorsTong-Xian Liu34
Estimated H-index: 34
(NWAFU: Northwest A&F University)
Abstract Insect cuticular hydrocarbons (CHCs), the evolutionary products of aquatic hexapod ancestors expanding to terrestrial environment, are deposited on the surface of insect integument and originally functioned primarily as waterproofing agents. CHCs are derived from the conserved fatty acid synthesis pathway in insects. However, the pivotal fatty acid synthase (FAS) involved in hydrocarbon (HC) biosynthesis remains unknown in many insect orders including the primitive Blattodea. Here, we i...
Published on Aug 21, 2019in bioRxiv
Amy Lynd11
Estimated H-index: 11
(LSTM: Liverpool School of Tropical Medicine),
Vasileia Balabanidou4
Estimated H-index: 4
(FORTH: Foundation for Research & Technology – Hellas)
+ 4 AuthorsGareth Lycett14
Estimated H-index: 14
(LSTM: Liverpool School of Tropical Medicine)
Oenocytes are an insect cell type having diverse physiological functions ranging from cuticular hydrocarbon (CHC) production to insecticide detoxification that may impact their capacity to transmit pathogens. To develop functional genetic tools to study Anopheles gambiae oenocytes, we have trapped an oenocyte enhancer to create a transgenic mosquito Gal4 driver line that mediates tissue-specific expression. After crossing with UAS-reporter lines, An. gambiae oenocytes are fluorescently tagged th...
Published on Jan 1, 2019in Frontiers in Physiology3.20
The functions of cuticular hydrocarbons (CHCs) are varied in insects, and one of them is to reduce water loss. Previous work has concluded that biosynthesis of cuticular hydrocarbons are strongly related to the CYP4G sub-family. Targeting these genes in the brown planthopper, Nilaparvata lugens Stal, might be a new application for integrated pest management. Therefore, we explored the functions of CYP4G76 (GenBank: KM217045.1) and CYP4G115 (GenBank: KM217046.1) genes in this study. The desiccati...
Published on 2019in Genome Biology and Evolution3.73
Cédric Finet11
Estimated H-index: 11
(École normale supérieure de Lyon),
Kailey Slavik (Harvard University)+ 2 AuthorsHenry Chung13
Estimated H-index: 13
(MSU: Michigan State University)
Published on May 1, 2019in Insect Biochemistry and Molecular Biology3.62
Dan-Ting Li1
Estimated H-index: 1
(ZJU: Zhejiang University),
Xuan Chen3
Estimated H-index: 3
(ZJU: Zhejiang University)
+ 2 AuthorsChuan-Xi Zhang28
Estimated H-index: 28
(ZJU: Zhejiang University)
Abstract The cuticular hydrocarbon (CHC) biosynthetic pathways branches off from the synthesis of fatty acids. Fatty acid elongases (ELOs) are enzymes catalyzing the synthesis of long-chain fatty acids and thereby contribute to the diversification of CHCs. Based on bioinformatics analyses we identified 20 ELO genes in the brown planthopper, Nilaparvata lugens . RNA interference against these genes demonstrated that 9 NlELO genes were essential for the survival of N. lugens nymphs and adults. Ind...
Published on Apr 1, 2019in Journal of Insect Physiology2.86
Xing-Xing Wang3
Estimated H-index: 3
(NWAFU: Northwest A&F University),
Zhu-Jun Feng (NWAFU: Northwest A&F University)+ 3 AuthorsTong-Xian Liu34
Estimated H-index: 34
(NWAFU: Northwest A&F University)
Abstract Melanism in insects is important for their physical protection, immunoreactions, and sclerotization. The vetch aphid, Megoura viciae (Buckton), has relatively strong tanning in its prothorax, head, antennae, cornicles, and legs. It was hypothesized that M. viciae may sequester the high level of l -DOPA in its host Vicia faba to help in its melanization process for ecological adaptation. To confirm this hypothesis, the amount of l -DOPA in M. viciae was modified and quantified. We first ...
Published on Feb 22, 2019in Science China-life Sciences3.58
Dan-Ting Li1
Estimated H-index: 1
(ZJU: Zhejiang University),
Xuan Chen1
Estimated H-index: 1
(ZJU: Zhejiang University)
+ 1 AuthorsChuan-Xi Zhang28
Estimated H-index: 28
(ZJU: Zhejiang University)
Many insects can live on water and survive being caught in the rain. Current research has shown that insect cuticular hydrocarbons (CHC) confer desiccation resistance to maintain water balance. In this study, we identified a fatty acyl-CoA reductase gene (NlFAR) of the rice brown planthopper, Nilaparvata lugens that is essential for the production of CHCs, and found that NlFAR is essential for N. lugens to walk and jump on water when moving from one rice plant to another in paddy fields. NlFAR w...
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