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The Reversal Effect and Its Mechanisms of Tetramethylpyrazine on Multidrug Resistance in Human Bladder Cancer.

Published on Jul 8, 2016in PLOS ONE 2.78
· DOI :10.1371/journal.pone.0157759
Shanshan Wang1
Estimated H-index: 1
Ting Lei1
Estimated H-index: 1
Man Zhang1
Estimated H-index: 1
Chemotherapy is an important strategy for the treatment of bladder cancer. However, the main problem limiting the success of chemotherapy is the development of multidrug resistance (MDR). To improve the management of bladder cancer, it is an urgent matter to search for strategies to reverse MDR. We chose three kinds of herbal medicines including ginsenoside Rh2, (-)-Epigallocatechin gallate (EGCG) and Tetramethylpyrazine (TMP) to detect their effects on bladder cancer. Reversal effects of these three herbal medicines for drug resistance in adriamycin (ADM)-resistant Pumc-91 cells (Pumc-91/ADM) were assessed by Cell Counting Kit-8 (CCK-8) cell proliferation assay system. The mechanisms of reversal effect for TMP were explored in Pumc-91/ADM and T24/DDP cells. After Pumc-91/ADM and T24/DDP cells were treated with TMP, cell cycle distribution analysis was performed by flow cytometry. The expression of MRP1, GST, BCL-2, LRP and TOPO-II was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR), immunefluorescence assay and western blot. It was observed that TMP was capable of enhancing the cytotoxicity of anticancer agents on Pumc-91/ADM cells in response to ADM, however Rh2 and EGCG were unable to. The reversal effect of TMP was also demonstrated in T24/DDP cells. Moreover, the treatment with TMP in Pumc-91/ADM and T24/DDP cells led to an increased of G1 phase accompanied with a concomitant decrease of cell numbers in S phase. Compared to the control group, an obvious decrease of MRP1, GST, BCL-2 and an increase of TOPO-II were shown in TMP groups with a dose-dependency in mRNA and protein levels. However, there was no difference on LRP expression between TMP groups and the control group. TMP could effectively reverse MDR of Pumc-91/ADM and T24/DDP cells and its mechanisms might be correlated with the alteration of MRP1, GST, BCL-2 and TOPO-II. TMP might be a potential candidate for reversing drug resistance in bladder cancer chemotherapy.
  • References (49)
  • Citations (5)
Published on Jan 1, 2015in CA: A Cancer Journal for Clinicians 223.68
Rebecca L. Siegel43
Estimated H-index: 43
(ACS: American Cancer Society),
Kimberly D. Miller16
Estimated H-index: 16
(ACS: American Cancer Society),
Ahmedin Jemal100
Estimated H-index: 100
(ACS: American Cancer Society)
Each year the American Cancer Society estimates the numbers of new cancer cases and deaths that will occur in the United States in the current year and compiles the most recent data on cancer incidence, mortality, and survival. Incidence data were collected by the National Cancer Institute (Surveillance, Epidemiology, and End Results [SEER] Program), the Centers for Disease Control and Prevention (National Program of Cancer Registries), and the North American Association of Central Cancer Regist...
Published on Jun 1, 2014in Experimental and Therapeutic Medicine 1.45
Fang Huang3
Estimated H-index: 3
(Fujian Medical University),
Xiaonan Wu4
Estimated H-index: 4
(Fujian Medical University)
+ 2 AuthorsZufu Lu20
Estimated H-index: 20
(USYD: University of Sydney)
Although its mechanisms remain unidentified, resveratrol (trans-3,4′,5-trihydroxystilbene; RES), which is an active, low molecular-weight compound, possesses a unique antitumor function and is capable of enhancing the cytotoxicity of doxorubicin (DOX) within solid tumor cells. RES is hypothesized to exert these effects by reversing the multidrug resistance (MDR) of the cancer cells in response to chemotherapeutic agents. The aim of the present study was to investigate the reversal effect of RES ...
Kyung Chan Kim7
Estimated H-index: 7
(DCU: Catholic University of Daegu),
ChuHee Lee15
Estimated H-index: 15
(Yeungnam University)
Lung cancer is still the number one cause of death from cancer worldwide. The clinical effect of platinum-based chemotherapy for non-small cell lung cancer is constrained by the resistance to drug. To overcome chemo-resistance, various modified treatment including combination therapy has been used, but overall survival has not been improved yet. In this study, chemo-resistant lung cancer cells, A549/Cis and H460/Cis, were developed by long-term exposure of cells to cisplatin and the proliferativ...
Published on Jan 1, 2014in Radiotherapy and Oncology 5.25
Hanxi Zhao4
Estimated H-index: 4
(Academy of Medical Sciences, United Kingdom),
W. Zhu3
Estimated H-index: 3
(Academy of Medical Sciences, United Kingdom)
+ 5 AuthorsLigang Xing1
Estimated H-index: 1
(Academy of Medical Sciences, United Kingdom)
Abstract Background and purpose Patients with unresectable stage III non-small-cell lung cancer receiving concurrent chemoradiotherapy often develop esophagitis that may lead to unplanned treatment interruptions, which may severely reduce rates of locoregional tumor control and survival. No effective treatment that would reduce the incidence and severity of this complication has been identified up to now. Although acceleration of normal tissue protection using epigallocatechin-3-gallate (EGCG) h...
Published on Aug 1, 2012in Biochemical and Biophysical Research Communications 2.71
Ning Xu6
Estimated H-index: 6
(SJTU: Shanghai Jiao Tong University),
Conghuan Shen5
Estimated H-index: 5
(SJTU: Shanghai Jiao Tong University)
+ 4 AuthorsJian-Jun Zhang9
Estimated H-index: 9
(SJTU: Shanghai Jiao Tong University)
Abstract Cisplatin is one of the commonly used chemotherapeutic drugs for the treatment of patients with advanced liver cancer. However, acquisition of cisplatin resistance is common in patients with hepatocellular carcinoma (HCC), and the underlying mechanism of such resistance is not fully understood. In the study, we found that miR-130a levels were significantly increased in HCC patients treated with cisplatin-based chemotherapy. miR-130a levels were also higher in cisplatin-resistant Huh7 ce...
Published on Jul 26, 2012in The New England Journal of Medicine 70.67
Paul Cathcart1
Estimated H-index: 1
Richard P. Steeds82
Estimated H-index: 82
James Catto51
Estimated H-index: 51
Published on Jun 1, 2011in BJUI 4.52
Jorge Rioja7
Estimated H-index: 7
(University of Navarra),
Eva Bandrés29
Estimated H-index: 29
(University of Navarra)
+ 9 AuthorsL. Plaza3
Estimated H-index: 3
What’s known on the subject? and What does the study add? SXR and MDR1 are known as responsible for chemo and radiotherapy resistance in some cancers, like kidney cancer (MDR1). Invasive bladder cancer is an aggressive disease, with different behaviour upon its tumoral stage, and also within the same tumoral stage, therefore molecular markers are sought. This study shows a new molecular marker, which has shown as a predictor for bad prognosis cancers, therefore, allowing us for a better patient ...
Published on Dec 1, 2010in Biochimica et Biophysica Acta 3.79
Beate Köberle17
Estimated H-index: 17
Maja T. Tomicic21
Estimated H-index: 21
+ 1 AuthorsBernd Kaina67
Estimated H-index: 67
Abstract Cisplatin is used for the treatment of many types of solid cancers. While testicular cancers respond remarkably well to cisplatin, the therapeutic efficacy of cisplatin for other solid cancers is limited because of intrinsic or acquired drug resistance. Our understanding about the mechanisms underlying cisplatin resistance has largely arisen from studies carried out with cancer cell lines in vitro . The process of cisplatin resistance appears to be multifactorial and includes changes in...
Published on Aug 1, 2010in Neoplasia 3.84
Andreas C. Hoffmann18
Estimated H-index: 18
(SC: University of Southern California),
Peter Wild45
Estimated H-index: 45
(ETH Zurich)
+ 8 AuthorsMartin Schuler49
Estimated H-index: 49
PURPOSE: The role of adjuvant chemotherapy in patients with locally advanced bladder cancer still remains to be defined. We hypothesized that assessing the gene expression of the chemotherapy response modifiers multidrug resistance gene 1 ( MDR1 ) and excision repair crosscomplementing 1 ( ERCC1 ) may help identify the group of patients benefiting from cisplatin-based adjuvant chemotherapy. EXPERIMENTAL DESIGN: Formalin-fixed paraffin-embedded tumor samples from 108 patients with locally advance...
Published on Feb 1, 2010in Journal of International Medical Research 1.35
Hongwei Xu6
Estimated H-index: 6
(SDU: Shandong University),
Lu Xu8
Estimated H-index: 8
(SDU: Shandong University)
+ 2 AuthorsLIUHongguo20
Estimated H-index: 20
(SDU: Shandong University)
This study evaluated the sensitivities of gastric cancer cells to various chemotherapy drugs, and investigated the relationship between the expression of P-glycoprotein (P-gp) and multidrug resistance-associated protein (MRP) and multidrug resistance. Drug sensitivities were determined using a methyltetrazolium assay: expression levels of P-gp and MRP were measured using immunohistochemistry. On purification culture, gastric cancer cells were found to be most sensitive to cisplatin, mitomycin an...
Cited By5
Published on Oct 1, 2018in Cancer Medicine 3.36
Wanru Ma , Juan Ma (PKU: Peking University)+ 3 AuthorsMan Zhang (PKU: Peking University)
Published on Jun 1, 2018in Chinese Journal of Integrative Medicine 1.45
Huandong Zhao1
Estimated H-index: 1
(CSU: Central South University),
Hong-juan Xie1
Estimated H-index: 1
(SJTU: Shanghai Jiao Tong University)
+ 2 AuthorsYuxiang Chen14
Estimated H-index: 14
(CSU: Central South University)
Multidrug resistance (MDR) is a major cause of cancer chemotherapy failure, and it is important to develop suitable reversal agents to overcome MDR. A majority of chemical reversal agents have acceptable reversal effects. However, the toxicity and adverse reactions associated with these agents restricts their clinical use. Chinese medicines (CMs) have lower toxicities and adverse reactions and are associated with multiple components, multiple targets and reduced toxicity. CMs have several advant...
Published on May 1, 2018in Bioorganic & Medicinal Chemistry 2.80
Yang Gao , Wei Shi6
Estimated H-index: 6
+ 6 AuthorsHai Qian17
Estimated H-index: 17
Abstract Multidrug resistance (MDR) is one of the main obstacles of clinical chemotherapy. A great deal of research shows that the occurrence of drug resistance in various malignant tumors is closely related to the expression of P-glycoprotein (P-gp) on the surface of the cell membrane. In this paper, based on the structure-activity relationship of phenylethyl tetrahydroisoquinoline, we choose tariquidar as the lead compound for the design and synthesis of 17 novel tetrahydroisoquinoline P-gp in...
Published on Jan 1, 2018in Technology in Cancer Research & Treatment 1.48
Xue Jiang (Capital Medical University), Ting Lei (Capital Medical University), Man Zhang3
Estimated H-index: 3
(Capital Medical University)
Objective:To explore the correlation of formyl peptide receptor 1 expression with drug resistance and the functions of formyl peptide receptor 1 in drug-resistant bladder cancer.Methods:Expression of formyl peptide receptor 1 in T24 and T24/DDP cisplatin-resistant bladder cancer cell lines was tested by quantitative real-time Polymerase Chain Reaction and Western blotting. After incubation of T24/DDP with N-formyl-Met-Leu-Phe, the phosphor proteins were tested by Western blot analysis. We charac...
Published on Jul 1, 2017in Apoptosis 4.02
Wei Lian3
Estimated H-index: 3
(Third Military Medical University),
Li Zhang1
Estimated H-index: 1
+ 1 AuthorsWensheng Chen10
Estimated H-index: 10
(Third Military Medical University)
Multidrug resistance (MDR) remains a major clinical obstacle in the treatment of gastric cancer (GC) since it causes tumor recurrence and metastasis. The transcription factor activator protein-2α (AP-2α) has been implicated in drug-resistance in breast cancer; however, its effects on MDR of gastric cancer are far from understood. In this study, we aimed to explore the effects of AP-2α on the MDR in gastric cancer cells selected by vincristine (VCR). Decreased AP-2α levels were markedly detected ...