Effect of acute skin thermal injury on subcutaneous glutathione, ascorbic acid and hydroxyl radical concentrations in anesthetized rats

SummaryThe effect of acute thermal injury on subcutaneous oxidative stress, in anesthetized rats, was evaluated. A microdialysis probe was implanted in the subcutaneous tissue for continuous sampling of interstitial fluids, and the microdialysates were injected onto either an on-line or an off-line high performance liquid chromatography system. Hydroxyl radicals, glutathione, and ascorbic acid concentrations in the microdialysates were analyzed....