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Comparison of CRISPR/Cas9 and TALENs on editing an integrated EGFP gene in the genome of HEK293FT cells

Published on Dec 1, 2016in SpringerPlus
· DOI :10.1186/s40064-016-2536-3
Zuyong He13
Estimated H-index: 13
(Edin.: University of Edinburgh),
Christopher Proudfoot11
Estimated H-index: 11
(Edin.: University of Edinburgh)
+ 1 AuthorsSimon G. Lillico24
Estimated H-index: 24
(Edin.: University of Edinburgh)
Abstract
Background Genome editors such as CRISPR/Cas9 and TALENs are at the forefront of research into methodologies for targeted modification of the mammalian genome. To date few comparative studies have been carried out to investigate the difference of genome editing characteristics between CRISPR/Cas9 and TALENs. While the CRISPR/Cas9 system has overtaken TALENs as the tool of choice for most research groups working in this field, we hypothesized that there could be certain applications whereby the application of TALENs would have specific benefits. Here we compare CRISPR/Cas9 and TALEN as tools for introducing site-specific editing events at an integrated EGFP gene in the genome of HEK293FT cells.
  • References (24)
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