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Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR

Published on Dec 1, 2016in Genome Biology14.028
· DOI :10.1186/s13059-016-1012-2
Maximilian Haeussler23
Estimated H-index: 23
(UCSC: University of California, Santa Cruz),
Kai Schönig22
Estimated H-index: 22
(Heidelberg University)
+ 9 AuthorsJean-Paul Concordet26
Estimated H-index: 26
(French Institute of Health and Medical Research)
Sources
Abstract
Background The success of the CRISPR/Cas9 genome editing technique depends on the choice of the guide RNA sequence, which is facilitated by various websites. Despite the importance and popularity of these algorithms, it is unclear to which extent their predictions are in agreement with actual measurements.
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