Different chromatographic methods for simultaneous determination of diloxanide furoate, metronidazole and its toxic impurity

TLC densitometric and RP-HPLC methods are innovative chromatographic methods used for determination of diloxanide furoate, metronidazole and its impurity, 4-nitroimidazole. In the developed TLC densitometric method, appropriate separation was achieved using silica gel 60 F254 TLC plates and ethyl acetate/acetone/hexane/ammonia solution (9.5:0.5:0.3:0.3, by volume), as a developing system and the separated bands were UV-scanned at 276 nm. While...