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agents and monoclonal antibody blockade platelet deposition on an artificial surface using clinical antiplatelet Examination of the roles of glycoprotein Ib and glycoprotein IIb/IIIa in

Published on Jan 1, 2011
Richard A. Sheppeck10
Estimated H-index: 10
,
Michael L. Bentz4
Estimated H-index: 4
+ 3 AuthorsJanine E. Janosky54
Estimated H-index: 54
Abstract
  • References (29)
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References29
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#1Hirofumi Nagai (Terumo)H-Index: 5
#2Makoto Handa (Keio: Keio University)H-Index: 38
Last. Yasuo Ikeda (Keio: Keio University)H-Index: 71
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Abstract We investigated the molecular mechanism(s) by which platelets adhere to an artificial surface exposed to plasma, using polystyrene microtiter plates pretreated with plasma. Washed platelets labelled with 51 Cr were incubated with the plates under static conditions. Prostaglandin E 1 (PGE 1 ) was added to the platelets to prevent platelet-platelet interactions. Adhesion required the presence of a divalent cation such as Mg ++ or Ca ++ . Polyclonal anti-fibrinogen antibody inhibited adhes...
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#1Tj Kunicki (Gulf Coast Regional Blood Center)H-Index: 23
The membrane glycoproteins (GP) of human platelets act as receptors that mediate two important functions, adhesion to the subendothelial matrix and platelet-platelet cohesion, or aggregation. Many of these glycoprotein receptors exist as noncovalently linked heterodimers, including those that belong to the supergene family of adhesion receptors called the integrins. Human platelets contain at least five members of this integrin family, including a collagen receptor (GP Ia-IIa; α2, β1), a fibrone...
36 CitationsSource
#1Barry S. Coller (SUNY: State University of New York System)H-Index: 8
#2Jürg Beer (SUNY: State University of New York System)H-Index: 44
Last. Martin H. Steinberg (SUNY: State University of New York System)H-Index: 73
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Using intact human platelets as the immunogen and a functional, collagen-coated bead agglutination assay, we have produced a murine monoclonal antibody (6F1) that blocks the interaction between platelets and collagen in the presence of Mg++. 6F1 affinity-purified the platelet glycoprotein Ia/IIa complex, and approximately 800 molecules of 6F1 bound per platelet at saturation. 6F1 nearly completely inhibited collagen-induced platelet aggregation and inhibited platelet adhesion to collagen by grea...
243 CitationsSource
Vascular subendothelium and collagenous surfaces were exposed to flowing citrated blood. Platelet interactions with these surfaces were investigated at various axial distances from the upstream end of the exposed surfaces. A pronounced axial decrease in surface coverage with platelets and in thrombus dimensions was encountered on collagenous surfaces. This phenomenon was observed at shear rates of 200 to 2000 s-1, but was most pronounced at low shear rates (less than 650 s-1). After 5 minutes of...
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#1Paul DidisheimH-Index: 5
#2Don B. OlsenH-Index: 29
Last. James M. AndersonH-Index: 79
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#1Andreas A. PootH-Index: 25
#2Tom BeugelingH-Index: 26
Last. W.G. van AkenH-Index: 24
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The capillary perfusion model according to Cazenave and co-workers was characterized by investigatingium labelling of human platelets and scanning electron microscopy (SEM). Compared with uncoated polyethylene, platelet deposition was increased after precoating with purified human von Willebrand factor, fibrinogen or fibronectin, and decreased by preadsorbed immunoglobulin G, albumin or whole plasma. Platelet aggregates were observed on immunoglobulin G-coated polyethylene, whereas all other sur...
31 CitationsSource
The interaction of platelets with natural and artificial surfaces is briefly reviewed, emphasizing the role of the platelet glycoprotein Ib and IIb/IIIa receptors. Studies utilizing monoclonal antibodies to these receptors for the diagnosis and therapy of hemorrhagic and thrombotic disorders are described, indicating the potential of such agents as platelet inhibitors.
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#1Jean McPhersonH-Index: 6
#2Sandra BrownleaH-Index: 2
Last. Marjorie B. ZuckerH-Index: 29
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The platelet retention test provides a measure of the number of platelets retained in a column of glass beads and is one of the few in vitro platelet function tests that is abnormal in von Willebrand's disease (vWd). In a two-stage test, 1 mL of blood (designated A) was passed through the column, followed by 5 mL of isotonic saline and then 5 mL of blood (B) in which platelet retention was measured. With normal blood as A and B, retention is very high in all 5 mL of blood B. In the first stage, ...
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