In vitro organogenesis and plant regeneration from stem segments of Torreya grandis

The organogenesis and proliferation of in vitro cultured stem segments of Chinese torreya(Torreya grandis cv.Merrillii) were studied.Results showed after inoculation pretreatment of the materials at 4 ℃ for 3 days,followed by exposure to weak light of 400 lx and cultured at 18 to 22 ℃ could effectively inhibit the browning of explants,which is the key step for success of culturing the explants.For inducing adventitious shoots the best medium was...