Membrane translocation and location in cells of red fluorescent protein fusion vector incorporated HIV-TAT protein

Abstract
Objective To construct the vector that expresses the fusion protein of HIV-Tat protein and red fluorescent protein(mCherry) in mammalian cells,and observe by fluorescence microscopy the intracellular transduction and localization of recombinant protein in cells,in order to obtain a useful tool for the study of the uptake mechanism and intracellular localization of HIV-TAT.Methods With the designed primer coding mCherry sequence,the mCherry gene...
Paper Details
Title
Membrane translocation and location in cells of red fluorescent protein fusion vector incorporated HIV-TAT protein
Published Date
Jan 1, 2009
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