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Dexamethasone Regulates Macrophage and Cd4+Cd25+ Cell Numbers in the Chicken Spleen

Published on Mar 1, 2016in Brazilian Journal of Poultry Science 0.61
· DOI :10.1590/18069061-2015-0035
Atilio Sersun Calefi7
Estimated H-index: 7
(USP: University of São Paulo),
W.M. Quinteiro-Filho11
Estimated H-index: 11
(USP: University of São Paulo)
+ 7 AuthorsJoão Palermo-Neto29
Estimated H-index: 29
(USP: University of São Paulo)
Cite
Abstract
Dexamethasone (DEX) is a corticoid hormone that is experimentally used to mimic the effects of increased levels of endogenous corticosterone observed during the stress response. Currently, stress is considered one of the major predisposing factors for diseases in the poultry industry. The aim of this study was to analyze the effects of DEX and/or of a 20-fold coccidial vaccine dose on leukocyte phenotypes in the spleen and cecal tonsils of chickens. Twenty specific-pathogen-free (SPF) Leghorn chickens were divided into four groups: a non-treated group (NT), a DEX-treated group (Dex), a vaccinated group (V) and a DEX-treated+vaccinated group (Dex+V). On experimental day (ED) 42, each bird in the vaccinated groups received a anti-coccidial vaccine. DEX was injected in the birds of the Dex and Dex+V groups (0.9 mg/kg) onED42 and ED45. The immunophenotyping was performed by flow cytometry analysis of splenocytes and cecal tonsils cells onED48. DEX treatment per se was unable to change CD4+CD8+, CD4+CD8+ and CD4-CD8+ populations with TCRgd or CD28 in the spleen, or macrophages and T lymphocytes in the cecal tonsils. V group birds presented higher numbers of splenic macrophages compared with those measured in the Dex+V group. The number of CD4+CD25+ cells in the spleen of birds of the V group was higher than those measured in the other experimental groups. Our data suggest that CD4+CD25+ cells and macrophages might be influenced by DEX treatment in spleen, but not in the cecal tonsils of chickens inoculated with Eimeria.
  • References (33)
  • Citations (4)
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References33
Newest
Published on Mar 1, 2015in Developmental and Comparative Immunology 3.12
Marie Luise Neulen2
Estimated H-index: 2
(LMU: Ludwig Maximilian University of Munich),
Birgit C. Viertlboeck17
Estimated H-index: 17
(LMU: Ludwig Maximilian University of Munich)
+ 1 AuthorsThomas W. Göbel28
Estimated H-index: 28
(LMU: Ludwig Maximilian University of Munich)
Abstract Chicken NK cells have been defined in embryonic spleen and intestinal epithelium as CD8 + lymphoid cells that lack BCR and TCR, whereas blood NK cells have not been phenotypically defined. Here we employed the mab, 8D12 directed against CHIR-AB1, a chicken Fc receptor, to define a previously uncharacterized lymphoid cell population in the blood. Although CHIR-AB1 expression was found on several cell populations, cells with extraordinary high CHIR-AB1 levels ranged between 0.4 and 2.8% i...
Published on Dec 1, 2013in Avian Diseases 1.31
G. R. Huff22
Estimated H-index: 22
,
W. E. Huff24
Estimated H-index: 24
,
N. C. Rath29
Estimated H-index: 29
SUMMARY We have studied the etiology of turkey osteomyelitis complex (TOC) for the past 20 yr and have determined that this syndrome is caused by the inability of some fast-growing male turkeys to cope with production stressors. Although immunosuppressive viruses have often been associated with susceptibility to gangrenous dermatitis (cellulitis), we hypothesize that production stressors alone can also undermine resistance to opportunistic pathogens by both increasing bacterial translocation fro...
Published on Nov 1, 2013in Developmental and Comparative Immunology 3.12
Ramesh K. Selvaraj11
Estimated H-index: 11
(Ohio Agricultural Research and Development Center)
Abstract Regulatory T cells (Tregs) are a subset of T cells that specialize in immune suppression. CD4 + CD25 + FoxP3 + T cells have been characterized as Tregs and extensively studied in mammals. In the absence of a putative FoxP3 ortholog in avians, CD4 + CD25 + cells is characterized as Tregs in avians. Avian CD4 + CD25 + cells produce high amounts of IL-10, TGF-β, CTLA-4, and LAG-3 mRNA; lack IL-2 mRNA; and suppress T cell proliferation in vitro through both contact-dependent and -independen...
Published on Mar 1, 2013in Developmental and Comparative Immunology 3.12
Lonneke Vervelde29
Estimated H-index: 29
(Edin.: University of Edinburgh),
Sylvia S. Reemers8
Estimated H-index: 8
(UU: Utrecht University)
+ 4 AuthorsChristine A. Jansen19
Estimated H-index: 19
(UU: Utrecht University)
Abstract Infection with highly pathogenic avian influenza (HPAI) in birds and mammals is associated with severe pathology and increased mortality. We hypothesize that in contrast to low pathogenicity avian influenza (LPAI) infection, HPAI infection of chicken dendritic cells (DC) induces a cytokine deregulation which may contribute to their highly pathogenic nature. Infection of DC with LPAI H7N1 and H5N2 resulted in viral RNA and NP expression without increase in time, in contrast to HPAI H7N1 ...
Published on Jan 1, 2013in Poultry Science 2.03
Revathi Shanmugasundaram10
Estimated H-index: 10
(Ohio Agricultural Research and Development Center),
Ramesh K. Selvaraj11
Estimated H-index: 11
(Ohio Agricultural Research and Development Center)
The CD4+CD25+ cells have T regulatory cell properties in chickens. This study investigated the effect of in ovo injection of anti-chicken CD25 monoclonal antibodies (0.5 mg/egg) on CD4+CD25+ cell depletion and on amounts of interleukin-2 mRNA and interferon-γ mRNA in CD4+CD25− cells posthatch. Anti-chicken CD25 or PBS (control) was injected into 16-d-old embryos. Chicks hatched from eggs injected with anti-chicken CD25 antibodies had a lower CD4+CD25+ cell percentage in the blood until 25 d post...
Published on Sep 1, 2012in Veterinary Immunology and Immunopathology 1.85
Revathi Shanmugasundaram10
Estimated H-index: 10
(Ohio Agricultural Research and Development Center),
Ramesh K. Selvaraj11
Estimated H-index: 11
(Ohio Agricultural Research and Development Center)
Abstract Thymic CD4 + CD25 + cells from ducks were characterized for mammalian T regulatory cells’ suppressive and cytokine production properties. The cross reactivity of anti-chicken CD25 monoclonal antibody with duck CD25 was confirmed by evaluating Concanavalin-A-stimulated CD25 upregulation in splenocytes. CD4 + CD25 + cells were detectable in the thymus, spleen, cecal tonsil, and lung (airsacs), but not in the bursa. Duck CD4 + CD25 + cells had approximately nine-fold higher IL-10 mRNA, 12-...
Published on Mar 27, 2012in PLOS ONE 2.78
Revathi Shanmugasundaram10
Estimated H-index: 10
,
Ramesh K. Selvaraj11
Estimated H-index: 11
(Ohio Agricultural Research and Development Center)
Thymic CD4+CD25+ cells have regulatory-T-cell-like properties in chickens. This study examined the ontogeny of CD4+CD25+ cells in the thymus and in peripheral compartments in chickens. CD4+CD25+ cells started to appear in the thymus at day 15 of incubation (E15), although at low percentages. Expressed as a percentage of CD4+ cells, CD4+CD25+ cells increased (P<0.01) from 1.7% at E20 to 7.3% at 0 d post-hatch (D0). CD4+CD25+ cells did not appear in the spleen or cecal tonsils of embryos. Expresse...
Published on Dec 1, 2011in Veterinary Immunology and Immunopathology 1.85
Sung Hyen Lee21
Estimated H-index: 21
(USDA: United States Department of Agriculture),
Hyun S. Lillehoj48
Estimated H-index: 48
(USDA: United States Department of Agriculture)
+ 8 AuthorsWongi Min16
Estimated H-index: 16
(Gyeongsang National University)
Abstract This study was carried out to develop and characterize mouse monoclonal antibodies (mAbs) against chicken CD25 ( ch CD25), the alpha chain of the interleukin-2 (IL-2) receptor. A recombinant chimeric ch CD25/IgG4 fusion protein was expressed in Chinese hamster ovary (CHO) cells and isolated from spent cell culture medium by protein G affinity chromatography. Purified ch CD25 protein was used to immunize mice, from which 54 stable hybridomas secreting ch CD25 mAbs were produced. Two mAbs...
Published on Jul 1, 2011in Poultry Science 2.03
Kyung-Woo Lee23
Estimated H-index: 23
(ARS: Agricultural Research Service),
Hyun S. Lillehoj48
Estimated H-index: 48
(ARS: Agricultural Research Service)
+ 2 AuthorsDong-Jun An16
Estimated H-index: 16
(South Korean Ministry for Food, Agriculture)
The increasing trends of legislative restrictions and voluntary removal of antibiotic growth promoters worldwide has already affected, and will continue to affect, poultry production and animal health. Necrotic enteritis (NE) is being considered among the most important infectious diseases in the current poultry production system globally, with an estimated annual economic loss of more than $2 billion, largely attributable to medical treatments and impaired growth performance. Thus, there is an ...
Published on Feb 15, 2011in Journal of Immunology 4.72
Revathi Shanmugasundaram10
Estimated H-index: 10
(Ohio Agricultural Research and Development Center),
Ramesh K. Selvaraj11
Estimated H-index: 11
(Ohio Agricultural Research and Development Center)
Chicken CD4+CD25+ cells were characterized for mammalian regulatory T cells’ suppressive and cytokine production properties. Anti-chicken CD25 mAb was produced in mice and conjugated with a fluorescent tag. The specificity of the Ab against chicken CD25 was confirmed by evaluating Con A-induced CD25 upregulation in thymocytes and by quantifying the CD25 mRNA content of positive and negative cells identified by anti-chicken CD25 Ab. The percentage of CD4+CD25+ cells, expressed as a percentage of ...
Cited By4
Newest
Published on Jul 15, 2019in Anatomia Histologia Embryologia 0.66
Mustafa Yıldız13
Estimated H-index: 13
(Çanakkale Onsekiz Mart University),
Isil Aydemir + 1 AuthorsUlker Eren (Adnan Menderes University)
Published on Apr 1, 2019in Research in Veterinary Science 1.75
Atilio Sersun Calefi7
Estimated H-index: 7
(USP: University of São Paulo),
Catarina Augusta de Queiroz Nunes (USP: University of São Paulo)+ 3 AuthorsJoão Palermo-Neto29
Estimated H-index: 29
(USP: University of São Paulo)
Abstract Heat stress has a relevant effect on animal health and productivity. Stress and environmental changes can contribute to disease development, such as avian necrotic enteritis (NE). The goal of this study was to analyze the effects of heat stress applied to broiler chickens in an experimental model of co-infection with Clostridium perfringens and Eimeria spp. Therefore, the current study was designed to analyze the effect of heat stress to broiler chickens in an experimental model of infe...
Published on Dec 18, 2018
Background. The spleen, as an important organ of immunogenesis, is sensitive to changes in the external and internal environment. This property is used in environmental biomonitoring. The value of the morphological studies of the spleen increases with diseases of birds, the pathogens of which constitute a threat to human life and health. They are necessary for qualitative and safe poultry production. Proceedings on the morphometric study of the spleen of birds are fragmentary in nature. Therefor...
Published on Sep 1, 2017in Worlds Poultry Science Journal 1.36
Atilio Sersun Calefi7
Estimated H-index: 7
,
W.M. Quinteiro-Filho11
Estimated H-index: 11
+ 1 AuthorsJoão Palermo-Neto29
Estimated H-index: 29
Published on Jun 1, 2017in Veterinary Immunology and Immunopathology 1.85
Barbora Bílková2
Estimated H-index: 2
(Charles University in Prague),
Zuzana Bainová1
Estimated H-index: 1
(Charles University in Prague)
+ 2 AuthorsMichal Vinkler13
Estimated H-index: 13
(Charles University in Prague)
Abstract While haematological variation is well known in birds, variation in avian breeds (distinct morphotypes of the same species) remains unexplored. Poultry breeds, in particular, may show interesting evolutionary patterns and economically-relevant physiological differences. We performed a comparative examination of blood cellular composition in five chicken ( Gallus gallus domesticus ) breeds: Araucana, Booted bantam, Czech, Minorca and Rosecomb bantam. In standard-environment-reared hens w...