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Virulence and antimicrobial resistance of Klebsiella pneumoniae isolated from passerine and psittacine birds

Published on Mar 3, 2016in Avian Pathology 1.96
· DOI :10.1080/03079457.2016.1142066
Yamê Miniero Davies1
Estimated H-index: 1
(USP: University of São Paulo),
Marcos Paulo Vieira Cunha6
Estimated H-index: 6
(USP: University of São Paulo)
+ 9 AuthorsTerezinha Knöbl12
Estimated H-index: 12
(USP: University of São Paulo)
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Abstract
ABSTRACTKlebsiella pneumoniae is considered one of the most important Gram-negative opportunistic pathogens. The contact between humans and birds poses health risks to both. The aim of this study was to investigate the resistance and virulence of K. pneumoniae isolates from psittacines and passerines, seized from illegal trade in Brazil. We analysed 32 strains isolated from birds of the orders Psittaciformes and Passeriformes by polymerase chain reaction (PCR) for virulence factor genes. Antibiotic resistance was assessed by disk diffusion assay and PCR. The results indicated that fimH (100%), uge (96.8%), kfu (81.2%) and irp-2 (68.7%) were the most common virulence genes, followed by kpn (46.8%), K2 (43.7%), mrkD (34.3%) and iroN (15.6%). The combination of virulence genes resulted in a great diversity of genotypes and the heterogeneity of the strains is also confirmed in the analysis by amplified fragment length polymorphism. The susceptibility profiles of the K. pneumoniae showed 25% of multiple antibi...
  • References (40)
  • Citations (6)
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References40
Newest
Published on Sep 1, 2014in International Journal of Infectious Diseases 3.54
Lays Katarina Lima Rocha1
Estimated H-index: 1
(UPE: Universidade de Pernambuco),
Rivaldo Lins dos Santos Neto1
Estimated H-index: 1
(UPE: Universidade de Pernambuco)
+ 3 AuthorsMárcia Maria Camargo de Morais7
Estimated H-index: 7
(UPE: Universidade de Pernambuco)
Summary Objectives Qnr-mediated quinolone resistance is increasingly detected worldwide, but few studies have been carried out so far in Brazil. The aim of this study was to test for qnr determinants in isolates of ciprofloxacin-resistant Klebsiella pneumoniae . Methods Fifteen ciprofloxacin-resistant isolates from urine cultures of hospitalized patients at a university hospital in North-East Brazil were investigated. Specific PCRs were performed for bla CTX-M and bla TEM , qnr , and class 1 int...
Published on Aug 1, 2014in F1000 Medicine Reports
Christopher A. Broberg9
Estimated H-index: 9
(UNC: University of North Carolina at Chapel Hill),
Michelle Palacios4
Estimated H-index: 4
(UNC: University of North Carolina at Chapel Hill),
Virginia L. Miller37
Estimated H-index: 37
Klebsiella pneumoniae is the causative agent of a variety of diseases, including pneumonia, urinary tract infections, septicemia, and the recently recognized pyogenic liver abscesses (PLA). Renewed efforts to identify and understand the bacterial determinants required to cause disease have come about because of the worldwide increase in the isolation of strains resistant to a broad spectrum of antibiotics. The recent increased isolation of carbapenem-resistant strains further reduces the availab...
Published on Jun 1, 2014in Pathogens and Global Health 1.97
Kamelia M. Osman11
Estimated H-index: 11
(Cairo University),
Hany M. Hassan2
Estimated H-index: 2
+ 1 AuthorsAhmed Abdelhafez2
Estimated H-index: 2
(Cairo University)
AbstractStudies on the prevalence and virulence genes of Klebsiella mastitis pathogens in a buffalo population are undocumented. Also, the association of rmpA kfu, uge, magA, Aerobactin, K1 and K2 virulent factors with K. pneumoniae buffalo, and cow mastitis is unreported. The virulence of K. pneumoniae was evaluated through both phenotypic and molecular assays. In vivo virulence was assessed by the Vero cell cytotoxicity, suckling mouse assay and mice lethality test. Antimicrobial susceptibilit...
Published on May 1, 2014in Emerging Infectious Diseases 7.18
Jonas Bonnedahl17
Estimated H-index: 17
,
Jorge Hernandez16
Estimated H-index: 16
+ 3 AuthorsMirva Drobni14
Estimated H-index: 14
Published on Jan 1, 2014in Emerging Infectious Diseases 7.18
Jonas Bonnedahl17
Estimated H-index: 17
,
Jorge Hernandez16
Estimated H-index: 16
+ 3 AuthorsMirva Drobni14
Estimated H-index: 14
Extended-Spectrum beta-Lactamases in Escherichia coli and Klebsiella pneumoniae in Gulls, Alaska, USA
Published on Dec 1, 2013in Journal of Medical Microbiology 1.93
H. Ciesielczuk5
Estimated H-index: 5
(QMUL: Queen Mary University of London),
Michael Hornsey15
Estimated H-index: 15
(QMUL: Queen Mary University of London)
+ 2 AuthorsDavid W. Wareham24
Estimated H-index: 24
(QMUL: Queen Mary University of London)
The objective of this study was to develop and validate an expanded multiplex PCR assay for the simultaneous detection of eight plasmid-mediated quinolone-resistance determinants in Enterobacteriaceae. Primers were designed to amplify conserved fragments of qnrABCDS, qepA, oqxAB and aac(6′)-Ib-cr genes and were optimized in uniplex and multiplex PCR assays with control template DNA. The assay was used to determine the prevalence of plasmid-mediated quinolone resistance (PMQR) genes in 174 ciprof...
Published on Oct 1, 2013in Pathologie Biologie
R. El Fertas-Aissani1
Estimated H-index: 1
,
Yamina Messai8
Estimated H-index: 8
+ 1 AuthorsRabah Bakour8
Estimated H-index: 8
Abstract Aim of the study To detect virulence factors in 54 Klebsiella pneumoniae isolates from different clinical specimens: urine (26), blood (11), pus (11), lung (four), cerebrospinal fluid (one) and ascitic fluid (one). Material and methods PCR was used to investigate virulence genes encoding adhesins ( fimH-1 , mrkD , kpn , ycfM ), siderophores ( entB : enterobactin, iutA : aerobactin, irp-1 , irp-2 , ybtS , fyuA : yersiniabactin, iroN : catechols receptor), protectines or invasins ( rmpA ,...
Published on Sep 1, 2013in Antimicrobial Agents and Chemotherapy 4.71
Federico Perez34
Estimated H-index: 34
,
Susan D. Rudin24
Estimated H-index: 24
(Case Western Reserve University)
+ 9 AuthorsDavid van Duin24
Estimated H-index: 24
(Cleveland Clinic)
Use of antibiotics among livestock contributes to the selection and dissemination of multidrug resistant (MDR) bacteria ([1][1]). Olaquindox and carbadox are quinoxaline derivatives with antibacterial properties that prevent dysentery and enhance weight gain in suckling pigs ([2][2]). Resistance to
Published on Mar 1, 2013in Journal of Zoo and Wildlife Medicine 0.52
Hilari Wanderley Hidasi1
Estimated H-index: 1
,
José Hidasi Neto1
Estimated H-index: 1
+ 3 AuthorsMaria Auxiliadora Andrade9
Estimated H-index: 9
Abstract: Enteric bacteria are considered important potential pathogens in avian clinical medicine, causing either primary or opportunistic infections. The aim of this study was to evaluate the frequency of enterobacteria in the intestinal microbiota of psittacine birds and to determine the antimicrobial susceptibility of the Escherichia coli isolates cultured. Fecal samples were collected from 300 parrots captured from the illegal wildlife trade in Goias, Brazil and were processed using convent...
Published on Sep 1, 2012in Pesquisa Veterinaria Brasileira 0.30
André Becker Simões Saidenberg7
Estimated H-index: 7
(USP: University of São Paulo),
Rodrigo Hidalgo Friciello Teixeira9
Estimated H-index: 9
+ 3 AuthorsNilson Roberti Benites9
Estimated H-index: 9
(USP: University of São Paulo)
Psittaciformes are one of the most endangered groups of birds, and several Brazilian species are classified between vulnerable and critically endangered. It is thus necessary to identify agents that cause infections in captive wild animals and to assess the risks posed thereof and to design interventions to minimize the possibility of disease outbreaks, leading to the conservation of endangered species. The purpose of this study was to identify enteropathogenic Escherichia coli (EPEC) cloacal is...
Cited By6
Newest
Published on Jul 24, 2019in Journal of Antimicrobial Chemotherapy 5.11
Xiao-Ping Liao15
Estimated H-index: 15
(SCAU: South China Agricultural University),
Run-Shi Yang7
Estimated H-index: 7
(SCAU: South China Agricultural University)
+ 11 AuthorsLu Han (SCAU: South China Agricultural University)
Published on Feb 1, 2019in Journal of Microbiology Immunology and Infection 2.46
Jiahui Li1
Estimated H-index: 1
,
Wenzi Bi2
Estimated H-index: 2
+ 5 AuthorsTieli Zhou2
Estimated H-index: 2
Abstract Background/purpose Trimethoprim-sulfamethoxazole (TMP-SMZ) is broadly administered to treat multiple infections, and the paucity of effective treatment alternatives for infections caused by Klebsiella pneumoniae has led to a renewed interest in TMP-SMZ. The aim of this study is to evaluate the antibacterial efficacy of TMP-SMZ against K. pneumoniae . Methods The resistance genes of K. pneumoniae clinical isolates were investigated by PCR, followed by conjugation experiments and multiloc...
Published on Jan 1, 2019in Journal of Exotic Pet Medicine 0.24
Belgi Diren Sigirci2
Estimated H-index: 2
(Istanbul University),
Baran Çelik1
Estimated H-index: 1
(Istanbul University)
+ 2 AuthorsSeyyal Ak13
Estimated H-index: 13
(Istanbul University)
Abstract Tetracycline is a broad-spectrum antibiotic which is commonly used in humans and animals for treatment of bacterial infections. Therefore, tetracycline-resistant Enterobacteriaceae species found in the nature, humans, and animals are usually considered a serious health concern. The feces of birds that live with humans may be a source for of these antibiotic resistant bacteria. For this reason, presence of tetracycline-resistant Enterobacteriaceae in bird droppings collected from 18 diff...
Published on Nov 21, 2018
Komkiew Pinpimai1
Estimated H-index: 1
(Massey University),
Wendi D. Roe9
Estimated H-index: 9
(Massey University)
+ 1 AuthorsKeren E. Dittmer11
Estimated H-index: 11
(Massey University)
ABSTRACT Klebsiella pneumoniae is a Gram-negative bacterium that may cause infection in a broad range of hosts. We report here the genome sequences of seven K. pneumoniae isolates from New Zealand sea lions.
Published on Nov 21, 2018
Komkiew Pinpimai1
Estimated H-index: 1
(Massey University),
Wendi D. Roe9
Estimated H-index: 9
(Massey University)
+ 2 AuthorsSarah Michael2
Estimated H-index: 2
(Massey University)
ABSTRACT Klebsiella pneumoniae is a Gram-negative bacterium that can be found in the environment, as well as on mucosal surfaces of humans and animals. Here, we report the genome sequence of five K. pneumoniae isolates from substrate samples and bird feces collected in the Subantarctic Islands of New Zealand.
Published on Oct 1, 2017in Brazilian Journal of Microbiology 2.86
Lilian Aparecida Sanches1
Estimated H-index: 1
(USP: University of São Paulo),
Marcelo da Silva Gomes4
Estimated H-index: 4
+ 5 AuthorsTerezinha Knöbl12
Estimated H-index: 12
(USP: University of São Paulo)
Abstract Psittacine birds have been identified as reservoirs of diarrheagenic Escherichia coli , a subset of pathogens associated with mortality of children in tropical countries. The role of other orders of birds as source of infection is unclear. The aim of this study was to perform the molecular diagnosis of infection with diarrheagenic E. coli in 10 different orders of captive wild birds in the state of Sao Paulo, Brazil. Fecal samples were analyzed from 516 birds belonging to 10 orders: Acc...
Published on May 16, 2017in Frontiers in Microbiology 4.26
Marta Piotrowska3
Estimated H-index: 3
(University of Warsaw),
Dominika Przygodzińska1
Estimated H-index: 1
(University of Warsaw)
+ 1 AuthorsMagdalena Popowska12
Estimated H-index: 12
(University of Warsaw)
Members of the genus Aeromonas that commonly occur in various aquatic ecosystems are taken into account as vectors spreading antibiotic resistance genes in the environment. In our study strains of Aeromonas spp. (n=104) not susceptible to ampicillin were isolated from municipal sewage of different levels of purification - raw sewage, activated sludge and treated wastewater. The crucial step of the study was the identification of β-lactamase resistance genes. The identified genes encode β-lactama...