Validation of formamide as a detubulation agent in isolated rat cardiac cells

Kawai M, Hussain M, and Orchard CH. Am J Heart Circ Physiol 277: H603–H609, 1999 developed a technique to detubulate rat ventricular myocytes using formamide and showed that detubulation results in a decrease in cell capacitance, Ca 2+ current density, and Ca 2+ transient amplitude. We have investigated the mechanism of this detubulation and possible direct effects of formamide. Staining ventricular cells with di-8-ANEPPS showed that the t...