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Promising approaches to crystallization of macromolecules suppressing the convective mass transport to the growing crystal

Published on Aug 31, 2015in Russian Chemical Reviews4.61
· DOI :10.1070/RCR4557
Konstantin M. Boyko8
Estimated H-index: 8
(RAS: Russian Academy of Sciences),
Vladimir O. Popov19
Estimated H-index: 19
(RAS: Russian Academy of Sciences),
M. V. Kovalchuk11
Estimated H-index: 11
(RAS: Russian Academy of Sciences)
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  • References (74)
  • Citations (9)
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References74
Newest
Published on Jun 1, 2015in Pharmacology & Therapeutics9.40
John D. McCorvy14
Estimated H-index: 14
(UNC: University of North Carolina at Chapel Hill),
Bryan L. Roth96
Estimated H-index: 96
(UNC: University of North Carolina at Chapel Hill)
Abstract Serotonin receptors are prevalent throughout the nervous system and the periphery, and remain one of the most lucrative and promising drug discovery targets for disorders ranging from migraine headaches to neuropsychiatric disorders such as schizophrenia and depression. There are 14 distinct serotonin receptors, of which 13 are G protein-coupled receptors (GPCRs), which are targets for approximately 40% of the approved medicines. Recent crystallographic and biochemical evidence has prov...
Published on Jan 1, 2015in Archiv Der Pharmazie2.15
Andrea Defant10
Estimated H-index: 10
,
Ines Mancini23
Estimated H-index: 23
+ 1 AuthorsJan Balzarini89
Estimated H-index: 89
(Rega Institute for Medical Research)
In search for more effective drugs against HIV infection acting as non-nucleoside reverse transcriptase inhibitors (NNRTIs), a series of new molecules with hybrid structures based on the natural product (+)-calanolide A and the synthetic molecule α-APA, known as potent and selective inhibitors of this enzyme, were selected by docking calculations. A convergent synthetic strategy gave 21 compounds with a 2H-pyran-2-one structural unit and bearing isosteric modifications, which were tested against...
Published on Sep 1, 2014in IUCrJ4.76
Michael Heymann14
Estimated H-index: 14
(Brandeis University),
Achini Opthalage1
Estimated H-index: 1
(Brandeis University)
+ 4 AuthorsSeth Fraden36
Estimated H-index: 36
(Brandeis University)
An emulsion-based serial crystallographic technology has been developed, in which nanolitre-sized droplets of protein solution are encapsulated in oil and stabilized by surfactant. Once the first crystal in a drop is nucleated, the small volume generates a negative feedback mechanism that lowers the supersaturation. This mechanism is exploited to produce one crystal per drop. Diffraction data are measured, one crystal at a time, from a series of room-temperature crystals stored on an X-ray semi-...
Published on Jul 1, 2014in Doklady Biochemistry and Biophysics0.61
Konstantin M. Boyko8
Estimated H-index: 8
(RAS: Russian Academy of Sciences),
M. A. Gorbacheva1
Estimated H-index: 1
(RAS: Russian Academy of Sciences)
+ 4 AuthorsVladimir O. Popov19
Estimated H-index: 19
(RAS: Russian Academy of Sciences)
The unidentified ligand, which is present in the crystal of the protein with unknown function STM4435 from Salmonella typhimurium, was identified using a combination of high-resolution X-ray crystallography and accurate-mass time-of-flight mass spectrometry. The identified glycerol was present as a component of the solutions used for the isolation and crystallization of the protein and serves as the ligand mimicking the natural metabolite, presumably, 2-keto-myo-isonitol, which is indicative of ...
Alexander McPherson53
Estimated H-index: 53
(UCI: University of California, Irvine),
Jose A. Gavira23
Estimated H-index: 23
(CSIC: Spanish National Research Council)
Protein crystallization was discovered by chance about 150 years ago and was developed in the late 19th century as a powerful purification tool and as a demonstration of chemical purity. The crystallization of proteins, nucleic acids and large biological complexes, such as viruses, depends on the creation of a solution that is supersaturated in the macromolecule but exhibits conditions that do not significantly perturb its natural state. Supersaturation is produced through the addition of mild p...
Published on Dec 13, 2013in PLOS ONE2.78
Michael D. Carruthers4
Estimated H-index: 4
,
Paul A. Nicholson1
Estimated H-index: 1
+ 1 AuthorsRobert S. Munson33
Estimated H-index: 33
Figures 1-3 are incorrect. The correct versions of Figures 1-3 can be viewed here: and
Published on Dec 1, 2013in FEBS Journal4.74
Richard Giegé53
Estimated H-index: 53
(CNRS: Centre national de la recherche scientifique)
Protein crystallization has been known since 1840 and can prove to be straightforward but, in most cases, it constitutes a real bottleneck. This stimulated the birth of the biocrystallogenesis field with both ‘practical’ and ‘basic’ science aims. In the early years of biochemistry, crystallization was a tool for the preparation of biological substances. Today, biocrystallogenesis aims to provide efficient methods for crystal fabrication and a means to optimize crystal quality for X-ray crystallo...
Published on Nov 1, 2013in Journal of Synchrotron Radiation
Sachiko Takahashi8
Estimated H-index: 8
,
Kazunori Ohta5
Estimated H-index: 5
(JAXA: Japan Aerospace Exploration Agency)
+ 9 AuthorsHiroshi Miyoshi2
Estimated H-index: 2
(JAXA: Japan Aerospace Exploration Agency)
The Japan Aerospace Exploration Agency (JAXA) started a high-quality protein crystal growth project, now called JAXA PCG, on the International Space Station (ISS) in 2002. Using the counter-diffusion technique, 14 sessions of experiments have been performed as of 2012 with 580 proteins crystallized in total. Over the course of these experiments, a user-friendly interface framework for high accessibility has been constructed and crystallization techniques improved; devices to maximize the use of ...
Published on May 31, 2013in International Journal of Molecular Sciences4.18
Irene Russo Krauss14
Estimated H-index: 14
(University of Naples Federico II),
Antonello Merlino29
Estimated H-index: 29
(University of Naples Federico II)
+ 1 AuthorsFilomena Sica17
Estimated H-index: 17
(University of Naples Federico II)
The elucidation of the three dimensional structure of biological macromolecules has provided an important contribution to our current understanding of many basic mechanisms involved in life processes. This enormous impact largely results from the ability of X-ray crystallography to provide accurate structural details at atomic resolution that are a prerequisite for a deeper insight on the way in which bio-macromolecules interact with each other to build up supramolecular nano-machines capable of...
Published on May 1, 2013in Crystallography Reports0.75
Konstantin M. Boyko8
Estimated H-index: 8
(RAS: Russian Academy of Sciences),
A. V. Lipkin8
Estimated H-index: 8
(Kurchatov Institute)
+ 1 AuthorsM. V. Kovalchuk8
Estimated H-index: 8
(Kurchatov Institute)
The Protein Factory was established at the Centre for Nano, Bio, Info, Cognitive, and Social Sciences and Technologies (NBICS Centre) of the National Research Centre “Kurchatov Institute” in 2010. The Protein Factory, together with the Centre for Synchrotron Radiation and Nanotechnology, promote research on structural biology. This paper presents the technology platforms developed at the Protein Factory and the facilities available for researchers. The main projects currently being performed at ...
Cited By9
Newest
M. A. Marchenkova7
Estimated H-index: 7
(RAS: Russian Academy of Sciences),
Petr V. Konarev23
Estimated H-index: 23
(RAS: Russian Academy of Sciences)
+ -3 AuthorsYurii V. Pisarevsky1
Estimated H-index: 1
(RAS: Russian Academy of Sciences)
AbstractThe pre-crystallization solution of the transaminase from Thermobaculum terrenum (TaTT) has been studied by small-angle X-ray scattering (SAXS). Regular changes in the oligomeric compositio...
Published on Nov 1, 2018in Crystallography Reports0.75
A. S. Boikova2
Estimated H-index: 2
(RAS: Russian Academy of Sciences),
Yu. A. D’yakova3
Estimated H-index: 3
(RAS: Russian Academy of Sciences)
+ 5 AuthorsM. V. Koval’chuk1
Estimated H-index: 1
(SPbU: Saint Petersburg State University)
Published on Nov 1, 2018in Crystallography Reports0.75
Yu. V. Kordonskaya1
Estimated H-index: 1
(RAS: Russian Academy of Sciences),
V. I. Timofeev7
Estimated H-index: 7
(RAS: Russian Academy of Sciences)
+ 4 AuthorsMikhail V. Kovalchuk2
Estimated H-index: 2
(SPbU: Saint Petersburg State University)
Published on Jun 6, 2018in Viruses3.81
N. N. Sykilinda12
Estimated H-index: 12
,
Alena Yu. Nikolaeva2
Estimated H-index: 2
+ 6 AuthorsKonstantin A. Miroshnikov15
Estimated H-index: 15
Proteins that include enzymatic domain degrading the bacterial cell wall and a domain providing transport through the bacterial outer membrane are considered as prospective compounds to combat pathogenic Gram-negative bacteria. This paper presents an isolation and study of an enzyme of this class naturally encoded in the prophage region of Acinetobacter baumannii AB 5075 genome. Recombinant protein expressed in E. coli exhibits an antimicrobial activity with respect to live cultures of Gram-nega...
Published on Nov 1, 2017in Crystallography Reports0.75
A. S. Boikova2
Estimated H-index: 2
(RAS: Russian Academy of Sciences),
Yu. A. D’yakova3
Estimated H-index: 3
(RAS: Russian Academy of Sciences)
+ 6 AuthorsM. V. Koval’chuk1
Estimated H-index: 1
(SPbU: Saint Petersburg State University)
The composition of lysozyme solutions in D2O under conditions favorable for the formation of tetragonal crystals has been investigated at different protein concentrations by small-angle X-ray scattering using the synchrotron radiation. In addition to lysozyme monomers, dimeric and octameric species are found in the crystallization solutions; the octamer content increases with an increase in the protein concentration. A comparison of the data with those obtained under similar conditions but with ...
Published on Jul 1, 2017in Small10.86
Alaa Adawy6
Estimated H-index: 6
(Radboud University Nijmegen),
Zakariae Amghouz11
Estimated H-index: 11
(University of Oviedo)
+ 1 AuthorsDaniela A. Wilson30
Estimated H-index: 30
(Radboud University Nijmegen)
The possibility of using sub-micrometer polymeric stomatocytes is investigated to effectuate confined crystallization of inorganic compounds. These bowl-shaped polymeric compartments facilitate confined crystallization while their glassy surfaces provide their crystalline cargos with convenient shielding from the electron beam's harsh effects during transmission electron microscopy experiments. Stomatocytes host the growth of a single nanocrystal per nanocavity, and the electron diffraction expe...
Published on Jul 1, 2017
A. S. Boikova2
Estimated H-index: 2
(RAS: Russian Academy of Sciences),
Yulia A. Dyakova4
Estimated H-index: 4
(RAS: Russian Academy of Sciences)
+ 8 AuthorsYurii V. Pisarevsky1
Estimated H-index: 1
(RAS: Russian Academy of Sciences)
Solutions of lysozyme in heavy water were studied by small-angle neutron scattering (SANS) at concentrations of 40, 20 and 10 mg ml−1 with and without the addition of precipitant, and at temperatures of 10, 20 and 30°C. In addition to the expected protein monomers, dimeric and octameric species were identified in solutions at the maximum concentration and close to the optimal conditions for crystallization. An optimal temperature for octamer formation was identified and both deviation from this ...
Published on Sep 1, 2016in Crystallography Reports0.75
Yu. A. Dyakova3
Estimated H-index: 3
(RAS: Russian Academy of Sciences),
M. A. Marchenkova7
Estimated H-index: 7
(RAS: Russian Academy of Sciences)
The possibilities of significantly improving the quality of planar systems based on photoactive porphyrin–fullerene dyads, layers based on cytochrome c and cardiolipin, and lysozyme crystals and films using a complex of in situ X-ray methods and simulation are described. The potential of X-ray phase-sensitive and surface-sensitive methods developed by M.V. Koval’chuk and researchers from his school in monitoring all stages of synthesis of partially ordered organic structure is demonstrated. This...
Published on Sep 1, 2016in Crystallography Reports0.75
Konstantin M. Boyko8
Estimated H-index: 8
(Kurchatov Institute),
Vladimir I. Timofeev2
Estimated H-index: 2
(Kurchatov Institute)
+ 3 AuthorsM. V. Koval’chuk3
Estimated H-index: 3
(Kurchatov Institute)
Conditions of mass transport to growing crystals have a considerable effect on the crystal size and quality. The reduction of convective transport can help improve the quality of crystals for X-ray crystallography. One approach to minimizing convective transport is crystallization in a microgravity environment, in particular, in space. The data obtained by our research team in protein crystallization experiments on the International Space Station are surveyed and analyzed.
Published on Apr 6, 2016in Crystal Growth & Design4.15
Mikhail Kovalchuk7
Estimated H-index: 7
,
A. E. Blagov7
Estimated H-index: 7
+ 6 AuthorsVladimir V. Volkov1
Estimated H-index: 1
From the base structure of tetragonal lysozyme crystals, the octamer cluster was selected as the possible element of crystal growth. The proposed model was verified by small-angle X-ray scattering (SAXS) measurements of lysozyme solutions. The results showed a noticeable presence of lysozyme dimers and octamers under crystallization conditions and the total absence of oligomers under conditions where crystal growth was impossible.