Visualization and quantification of monoallelic TCRα gene rearrangement in αβ T cells

T‐cell receptor α (TCRα) chain rearrangement is not constrained by allelic exclusion and thus αβ T cells frequently have rearranged both alleles of this locus. Thereby, stepwise secondary rearrangements of both TCRα loci further increase the odds for generation of an α‐chain that can be positively selected in combination with a pre‐existing TCRβ chain. Previous studies estimated that approximately 2–12% of murine and human αβ T cells still carry...