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Development of compartment for studies on the growth of protein crystals in space

Published on Mar 1, 2016in Review of Scientific Instruments 1.59
· DOI :10.1063/1.4942961
Tomoya Yamazaki6
Estimated H-index: 6
,
Katsuo Tsukamoto21
Estimated H-index: 21
+ 6 AuthorsYuki Kimura13
Estimated H-index: 13
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Abstract
To clarify the growth mechanism of a protein crystal, it is essential to measure its growth rate with respect to the supersaturation. We developed a compartment (growth cell) for measuring the growth rate (<0.1 nm s−1) of the face of a protein crystal at a controlled supersaturation by interferometry over a period of half a year in space. The growth cell mainly consists of quartz glass, in which the growth solution and a seed crystal are enclosed by capillaries, the screw sample holder, and a helical insert. To avoid the destruction of the cell and the evaporation of the water from the solution inside the cell, we selected the materials for these components with care. The equipment was successfully used to examine the growth of a lysozyme crystal at a controlled supersaturation in space, where convection is negligible because of the microgravity environment, thereby advancing our understanding of the mechanism of protein crystal growth from solution. The technique used to develop the growth cell is useful...
  • References (25)
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References25
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Published on Oct 7, 2015in Crystal Growth & Design 4.15
Yoshihisa Suzuki15
Estimated H-index: 15
,
Katsuo Tsukamoto21
Estimated H-index: 21
+ 2 AuthorsTakahisa Fujiwara4
Estimated H-index: 4
The normal growth rates R and apparent step velocities (lateral growth rates of a spiral hillock) V of tetragonal hen egg-white lysozyme (HEWL) crystals were for the first time measured by Michelson interferometry in the international space station (as part of the NanoStep project) using commercialized HEWL samples containing 1.5% impurities. A significant increase in V under microgravity was confirmed compared to step velocities Vstep on the ground, while a decrease in R was also confirmed comp...
Published on Aug 1, 2015in Review of Scientific Instruments 1.59
Takahisa Fujiwara4
Estimated H-index: 4
(University of Tokushima),
Yoshihisa Suzuki15
Estimated H-index: 15
(University of Tokushima)
+ 9 AuthorsTomoya Yamazaki6
Estimated H-index: 6
(Tohoku University)
The normal growth rates of the {110} faces of tetragonal hen egg-white lysozyme crystals, R, were measured as a function of the supersaturation σ parameter using a reflection type interferometer under μG at the International Space Station (NanoStep Project). Since water slightly evaporated from in situ observation cells during a long-term space station experiment for several months, equilibrium temperature Te changed, and the actual σ, however, significantly increased mainly due to the increase ...
Published on May 1, 2014in Crystal Research and Technology 1.09
Kenta Murayama4
Estimated H-index: 4
(Tohoku University),
Katsuo Tsukamoto21
Estimated H-index: 21
(Tohoku University)
+ 3 AuthorsYuki Kimura3
Estimated H-index: 3
(Tohoku University)
The two-dimensional (2D) distributions of surface supersaturation of sodium chlorate crystals with and without solutal convection have been measured by means of a multidirectional interferometry (MDI) technique coupled with the principles of three-dimensional (3D) computer tomography. When solutal convection was present over a top face, the supersaturation at the center of the face was depleted by a factor of >0.9 with reference to the value at the edges of the crystal. When the convection was s...
Published on Oct 1, 2013in Review of Scientific Instruments 1.59
Izumi Yoshizaki14
Estimated H-index: 14
,
Katsuo Tsukamoto21
Estimated H-index: 21
+ 6 AuthorsMasaru Tachibana18
Estimated H-index: 18
The growth rate vs. supersaturation of a lysozyme crystal was successfully measured in situ together with the crystal surface observation and the concentration measurements onboard the International Space Station. A Michelson-type interferometer and a Mach-Zehnder interferometer were, respectively, employed for real-time growth rate measurements and concentration field measurements. The hardware development, sample preparation, operation, and analysis methods are described.
Published on Aug 1, 2013in Journal of Crystal Growth 1.57
Weichun Pan4
Estimated H-index: 4
(ZJSU: Zhejiang Gongshang University),
Jin Xu2
Estimated H-index: 2
(Wenzhou University)
+ 5 AuthorsYuying Fu1
Estimated H-index: 1
(ZJSU: Zhejiang Gongshang University)
Abstract Motivated by the enhancement of protein quality under microgravity condition, the behaviors of crystal growth under various gravity conditions have been monitored via Foton Satellite and parabolic flight. We found that the normal growth rate and the step velocity would be enhanced only at high protein concentration. Although the difference of diffusion between monomer lysozyme molecule and main impurity species in HWEL dimer may be able to explain this enhancement in long period at high...
Hiroaki Nakano13
Estimated H-index: 13
(Hyogo University of Health Sciences),
Allin Hosokawa2
Estimated H-index: 2
(Kyoto Prefectural University)
+ 5 AuthorsKunihiko Watanabe15
Estimated H-index: 15
(Kyoto Prefectural University)
Pz peptidase B is an intracellular M3 metallopeptidase that is found together with Pz peptidase A in the thermophile Geobacillus collagenovorans MO-1 and recognizes collagen-specific tripeptide units (-Gly-Pro-X-). These peptidases have low homology in their primary structures; however, their cleavage patterns towards peptide substrates are similar. In this work, Pz peptidase B was crystallized using the counter-diffusion method. Data were collected to a resolution of 1.6 A at 100 K from a cryst...
A Van Driessche6
Estimated H-index: 6
,
Juan Manuel García-Ruiz32
Estimated H-index: 32
+ 2 AuthorsHisao Satoh11
Estimated H-index: 11
Abstract Mineralogical processes taking place close to equilibrium, or with very slow kinetics, are difficult to quantify precisely. The determination of ultraslow dissolution/precipitation rates would reveal characteristic timing associated with these processes that are important at geological scale. We have designed an advanced high-resolution white-beam phase-shift interferometry microscope to measure growth rates of crystals at very low supersaturation values. To test this technique, we have...
Published on Jun 1, 2011in Crystal Growth & Design 4.15
Koji Inaka20
Estimated H-index: 20
,
Sachiko Takahashi8
Estimated H-index: 8
+ 10 AuthorsTomoyuki Kobayashi19
Estimated H-index: 19
Lipocalin-type prostaglandin (PG) D synthase (L-PGDS) catalyzes the isomerization of PGH2 to PGD2 and is involved in the regulation of pain and of nonrapid eye movement sleep and the differentiation of male genital organs and adipocytes, etc. L-PGDS is secreted into various body fluids and binds various lipophilic compounds with high affinities, acting also as an extracellular transporter. Mouse L-PGDS with a C65A mutation was previously crystallized with citrate or malonate as a precipitant, an...
Published on Dec 3, 2008in Crystal Growth & Design 4.15
Luis Antonio González-Ramírez8
Estimated H-index: 8
,
Jordi Carrera1
Estimated H-index: 1
+ 2 AuthorsJuan Manuel García-Ruiz32
Estimated H-index: 32
6 pages, 3 figures.-- Supporting information (Video animation of the FOTON M-3 Mission and Photographs showing two views of the GCF) available at: http://pubs.acs.org/doi/suppl/10.1021/cg800788e
Published on Dec 1, 2007in Applied Geochemistry 2.89
M. Sorai1
Estimated H-index: 1
(AIST: National Institute of Advanced Industrial Science and Technology),
T. Ohsumi1
Estimated H-index: 1
+ 1 AuthorsK. Tsukamoto1
Estimated H-index: 1
(Tohoku University)
Abstract To assess CO 2 underground sequestration from a geochemical viewpoint, the anorthite dissolution rate, which is an important parameter of risk analysis, was measured in a CO 2 –water system. The authors sought to obtain precise dissolution rate data in a short time observing a crystal surface on a nanoscale. For this purpose, phase-shift interferometry was applied. Using this method, uncertainty of the reactive surface area that is imparted on calculation of the dissolution rate constan...
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