High-Throughput Thermal Scanning for Protein Stability: Making a Good Technique More Robust

Volume: 15, Issue: 8, Pages: 387 - 392
Published: Jun 10, 2013
Abstract
We present a high-throughput approach to help define experimental formulations that enhance protein stability, which is based on differential scanning fluorimetry (DSF). The method involves defining the thermal stability of a protein against a screen of 13 buffer systems, systematically sampling pH from 5.0 to 9.0 at high and low salt concentrations, using both redundancy and extensive controls to make the method robust. The screen allows rapid...
Paper Details
Title
High-Throughput Thermal Scanning for Protein Stability: Making a Good Technique More Robust
DOI
Published Date
Jun 10, 2013
Journal
Volume
15
Issue
8
Pages
387 - 392
Citation AnalysisPro
  • Scinapse’s Top 10 Citation Journals & Affiliations graph reveals the quality and authenticity of citations received by a paper.
  • Discover whether citations have been inflated due to self-citations, or if citations include institutional bias.