Abstract
Clustered regularly interspaced short palindromic repeats-CRISPR associated nuclease 9 (CRISPR-Cas9) is a genome editing tool derived from microbial adaptive immune defense system.1 The nuclease Cas9 can generate double-strand breaks (DSBs) on the target DNA sequences in a site-specific way directed by a singly guide RNA (sgRNA) upon the existence of an pro-tospacer adjacent motif (PAM) sequence (Figure 1a).2, 3, 4 The resultant DSDs are...
Paper Details
Title
In vivo gene therapy potentials of CRISPR-Cas9
Published Date
Mar 31, 2016
Journal
Volume
23
Issue
7
Pages
557 - 559
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Notes
History