Original paper
In-Situ Observation of Membrane Protein Folding during Cell-Free Expression
Abstract
Proper insertion, folding and assembly of functional proteins in biological membranes are key processes to warrant activity of a living cell. Here, we present a novel approach to trace folding and insertion of a nascent membrane protein leaving the ribosome and penetrating the bilayer. Surface Enhanced IR Absorption Spectroscopy selectively monitored insertion and folding of membrane proteins during cell-free expression in a label-free and...
Paper Details
Title
In-Situ Observation of Membrane Protein Folding during Cell-Free Expression
Published Date
Mar 15, 2016
Journal
Volume
11
Issue
3
Pages
e0151051 - e0151051
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Notes
History