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Neuroprotective effects of adenosine isolated from Cordyceps cicadae against oxidative and ER stress damages induced by glutamate in PC12 cells

Published on Jun 1, 2016in Environmental Toxicology and Pharmacology3.06
· DOI :10.1016/j.etap.2016.02.009
Opeyemi J. Olatunji4
Estimated H-index: 4
(Jiangsu University),
Yan Feng3
Estimated H-index: 3
(Jiangsu University)
+ 5 AuthorsXiaofeng Yu2
Estimated H-index: 2
(Jiangsu University)
Cite
Abstract
Abstract Glutamate has been proven to induce oxidative stress through the formation of reactive oxygen species (ROS) and increased calcium overload which results in neuronal injury, development of neurodegenerative diseases and death. Adenosine is one of the bioactive nucleosides found in Cordyceps cicadae and it has displayed several pharmacological activities including neuroprotection. In this study, the protective effects of adenosine from C. cicadae against glutamate-induce oxidative stress in PC12 cells were evaluated. The exposure of PC12 cells to glutamate (5 mM) induced the formation of ROS, increased Ca 2+ influx, endoplasmic reticulum (ER) stress and up regulated the expression of pro-apoptotic factor Bax. However, pretreatment with adenosine markedly increased cell viability, decreased the elevated levels of ROS and Ca 2+ induced by glutamate. Furthermore adenosine increased the activities of GSH-Px and SOD, as well as retained mitochondria membrane potential (MMP), increased Bcl-2/Bax ratio, and reduced the expression of ERK, p38, and JNK. Overall, our results suggest that adenosine may be a promising potential therapeutic agent for the prevention and treatment of neurodegenerative disorders.
  • References (46)
  • Citations (13)
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References46
Newest
Published on Oct 23, 2015in Journal of Natural Products4.26
Meng-Ying Lu1
Estimated H-index: 1
,
Chin-Chu Chen7
Estimated H-index: 7
+ 2 AuthorsChia-Feng Kuo2
Estimated H-index: 2
Natural products play an important role in promoting health with relation to the prevention of chronic inflammation. N6-(2-Hydroxyethyl)adenosine (HEA), a physiologically active compound in the medicinal mushroom Cordyceps cicadae, has been identified as a Ca2+ antagonist and shown to control circulation and possess sedative activity in pharmacological tests. The fruiting body of C. cicadae has been widely applied in Chinese medicine. However, neither the anti-inflammatory activities of HEA nor ...
Published on May 14, 2014in Molecules3.06
Wen Bo Zeng1
Estimated H-index: 1
(Yunnan University),
Hong Yu3
Estimated H-index: 3
(Yunnan University)
+ 5 AuthorsAlison Adams1
Estimated H-index: 1
A rapid HPLC method had been developed and used for the simultaneous determination of 10 nucleosides (uracil, uridine, 2'-deoxyuridine, inosine, guanosine, thymidine, adenine, adenosine, 2'-deoxyadenosine and cordycepin) in 10 populations of Cordyceps cicadae, in order to compare four populations of Ophicordyceps sinensis and one population of Cordyceps militaris. Statistical analysis system (SAS) 8.1 was used to analyze the nucleoside data. The pattern of nucleoside distribution was analyzed in...
Published on Feb 1, 2014in Phytomedicine4.18
Rong Zhu3
Estimated H-index: 3
(SHU: Shanghai University),
Rong Zheng3
Estimated H-index: 3
(SHU: Shanghai University)
+ 2 AuthorsShuwei Zhang1
Estimated H-index: 1
(CAS: Chinese Academy of Sciences)
Abstract Chronic kidney disease is a growing public health problem with an urgent need for new pharmacological agents. Ergosterol peroxide (EP) is the major sterol produced by Cordyceps cicadae Shing ( C. cicadae ), a widely used traditional Chinese medicine. C. cicadae has been used to treat many kinds of diseases and has a potential benefit on renoprotection. This study aimed to investigate the anti-fibrotic effects of EP as well as the underlying mechanisms. A normal rat kidney fibroblast cel...
Published on Jan 1, 2014in Neurochemistry International3.99
Xiaohua Han3
Estimated H-index: 3
(Qingdao University),
Shaolei Zhu1
Estimated H-index: 1
(Qingdao University)
+ 4 AuthorsZhiqiang Qu4
Estimated H-index: 4
(Qingdao University)
Abstract Oxidative stress-induced neuronal death plays a pivotal role in pathogenesis of neurodegenerative disorders. Recently, 7,8-dihydroxyflavone (7,8-DHF) has been shown to exert neuroprotective effects by acting as a selective tyrosine kinase receptor B (TrkB) agonist. In addition, the antioxidant action of 7,8-DHF may protect neuronal cells against oxidative injury. In the present study, we used PC12 cells, a cell line generally thought to lack TrkB, to investigate the effect of 7,8-DHF on...
Published on Jan 1, 2014in Environmental Toxicology and Pharmacology3.06
Yukari Horio1
Estimated H-index: 1
(Hokkaido University),
Yongkun Sun5
Estimated H-index: 5
(Hokkaido University)
+ 2 AuthorsMasaaki Kurasaki22
Estimated H-index: 22
(Hokkaido University)
Abstract Aspartame is an artificial sweetner added to many low-calorie foods. The safety of aspartame remains controversial even though there are many studies on its risks. In this study, to understand the physiological effects of trace amounts of artificial sweetners on cells, the effects of aspartame on apoptosis were investigated using a PC12 cell system. In addition, the mechanism of apoptosis induced by aspartame in PC12 cells and effects on apoptotic factors such as cytochrome c, apoptosis...
Published on Dec 27, 2012in Molecules3.06
Meng-Shiou Lee16
Estimated H-index: 16
,
Jung Chao8
Estimated H-index: 8
+ 5 AuthorsHao-Yuan Cheng12
Estimated H-index: 12
Glutamate-induced excitotoxicity has been implicated in a variety of neuronal degenerative disorders. In the present study, we investigated the possible neuroprotective effects of schizandrin against apoptosis of primary cultured rat cortical cells induced by glutamate. Glutamate (10 μM) administered for 24 h decreased the expression of Bcl-2 and Bcl-XL protein, whereas increased the expression of Bax, Bak, apoptosis inducing factor (AIF), endonuclease G (Nodo G) and endoplasmic reticulum (ER) s...
Published on Dec 1, 2011in Neurobiology of Aging4.40
Jindong Xu4
Estimated H-index: 4
(ISMMS: Icahn School of Medicine at Mount Sinai),
Maria Xilouri21
Estimated H-index: 21
(Academy of Athens)
+ 5 AuthorsNikolaos K. Robakis43
Estimated H-index: 43
(ISMMS: Icahn School of Medicine at Mount Sinai)
Abstract To reduce damage from toxic insults such as glutamate excitotoxicity and oxidative stresses, neurons may deploy an array of neuroprotective mechanisms. Recent reports show that progranulin ( PGRN ) gene null or missense mutations leading to inactive protein, are linked to frontotemporal lobar degeneration (FTLD), suggesting that survival of certain neuronal populations needs full expression of functional PGRN. Here we show that extracellular PGRN stimulates phosphorylation/activation of...
Published on Jul 1, 2011in Journal of Medicinal Food2.02
Hyung Sook Kim11
Estimated H-index: 11
,
Jee Youn Kim12
Estimated H-index: 12
+ 7 AuthorsSang-Bae Han36
Estimated H-index: 36
Abstract Paecilomyces cicadae Miquel Samson is the anamorph of Cordyceps cicadae Shing and is used in functional foods for the prevention and treatment of various diseases. In the present study, we examined the effects of P. cicadae polysaccharide (PCP) on dendritic cell (DC) maturation. Phenotypic maturation of DCs by PCP was confirmed by the elevated expressions of CD80, CD86, major histocompatibility complex (MHC)-I, and MHC-II molecules and functional maturation by increased expression of in...
Published on May 1, 2011in Phytotherapy Research3.77
Wenbin Shen1
Estimated H-index: 1
(NU: Nanjing University),
Dan Song1
Estimated H-index: 1
(NU: Nanjing University)
+ 1 AuthorsWeiyun Zhang4
Estimated H-index: 4
(NU: Nanjing University)
Cordyceps sinensis as a well-known traditional Chinese tonic has many therapeutic functions. In the present study, an acid polysaccharide (APS) was isolated from cultivated Cordyceps mycelia by ion-exchange and sizing chromatography. The protective capacity of APS against H2O2-induced oxidative damage in rat pheochromocytoma PC12 cells was investigated by measuring cell viability, lactate dehydrogenase (LDH) release, antioxidant enzyme activity, malondialdehyde (MDA) levels and intracellular acc...
Published on Mar 1, 2011in Journal of Ethnopharmacology3.41
Zenji Kawakami10
Estimated H-index: 10
,
Hitomi Kanno12
Estimated H-index: 12
+ 1 AuthorsYoshio Kase30
Estimated H-index: 30
Abstract Aim of the study Yokukansan is a traditional Japanese medicine consisted of seven medicinal herbs and has been used for treatment of neurosis, insomnia, and behavioral and psychological symptoms of dementia in Japan. The aim of the present study is to clarify the active compounds responsible for the protective effect of yokukansan against glutamate-induced cytotoxicity in PC12 cells. Materials and methods PC12 cells which is a tool for selective evaluation of test substances against oxi...
Cited By13
Newest
Published on Aug 23, 2019
Dahae Lee11
Estimated H-index: 11
,
Won-Yung Lee + 6 AuthorsKi Sung Kang1
Estimated H-index: 1
Cordyceps militaris is a well-known medicinal mushroom. It is non-toxic and has clinical health benefits including cancer inhibition. However, the anticancer effects of C. militaris cultured in brown rice on breast cancer have not yet been reported. In this study, we simultaneously investigated the anticancer effects of cordycepin and an extract of C. militaris cultured in brown rice on MCF-7 human breast cancer cells using a cell viability assay, cell staining with Hoechst 33342, and an image-b...
Yanfang Sun3
Estimated H-index: 3
(ZSTU: Zhejiang Sci-Tech University),
Eva Kmonickova + 6 AuthorsHuigang Wang9
Estimated H-index: 9
(ZSTU: Zhejiang Sci-Tech University)
Abstract Cordyceps cicadae is an entomogenous fungus that has been used as a valuable traditional Chinese herbal tonic, however, it can be difficult to discern the false from the genuine. In this study, the macroscopic IR fingerprint methods containing Fourier transform infrared spectroscopy (FT-IR) and second derivative infrared spectroscopy (SD-IR) were used to elucidate wild C. cicadae . The TOPSIS (Technique for Order Preference by Similarity to Ideal Solution) method was used to comprehensi...
Published on Jan 1, 2019
Jacqueline A. Takahashi21
Estimated H-index: 21
(UFMG: Universidade Federal de Minas Gerais),
Denise Sande (UFMG: Universidade Federal de Minas Gerais)+ 2 AuthorsMatheus Thomaz Nogueira Silva Lima1
Estimated H-index: 1
(UFMG: Universidade Federal de Minas Gerais)
Abstract Alzheimer's disease (AD), discovered by Alois Alzheimer in the early 1900s, is a complex and progressive neurodegenerative disorder, characterized by episodes of memory loss, singled out as the most common cause of dementia in the elderly Wolk et al. (2017) [1] . From a molecular point of view, the aggregation of amyloid-β peptides into fibrils is central to the pathogenesis Qiang et al. (2017) [2] . Cognitive decline observed in AD patients is also hypothesized to be related to the red...
Ying Chen7
Estimated H-index: 7
(ZJU: Zhejiang University),
Lifeng Fu + 5 AuthorsXiangnan Zhang12
Estimated H-index: 12
(ZJU: Zhejiang University)
Corbrin Capsule, a preparation of Cordyceps sinensis analogue, is a pleiotropic traditional Chinese patent medicine with the main component of fermentative cordyceps fungus powder (Cs-C-Q80). The neuroprotective effects of Cs-C-Q80, as a substitution of Cordyceps sinensis, have not been fully identified. The objectives of this study were to explore the prophylactic and therapeutic effects of Cs-C-Q80 in vascular dementia mice model. The efficacy of Cs-C-Q80 was investigated in a molecular level ...
Published on Dec 1, 2018in Scientific Reports4.01
Maslin Osathanunkul7
Estimated H-index: 7
(CMU: Chiang Mai University),
Khukrit Osathanunkul (Payap University)+ 2 AuthorsPanagiotis Madesis20
Estimated H-index: 20
Bar-HRM is a hybrid method which combines DNA barcoding and High Resolution Melting analysis. It has proven to be a fast, cost-effective and reliable molecular approach for species identification and authentication. Here, three aspects of the use of Bar-HRM are focused on. First, Bar-HRM is used to discriminate between closely related Ophiocordyceps species. Second, identification of an unknown powder that is claimed to be Ophiocordyceps species using Bar-HRM. Third, authenticating the O. sinens...
Published on Sep 1, 2018in Fitoterapia2.43
Opeyemi Joshua Olatunji3
Estimated H-index: 3
(PSU: Prince of Songkla University),
Jian Tang4
Estimated H-index: 4
(Jiangsu University)
+ 3 AuthorsZhen Ouyang5
Estimated H-index: 5
(Jiangsu University)
Abstract The genus Cordyceps has about 750 identified species which are distributed in many regions of the World, but mainly found in South Asia, Europe and North America. The species of the genus are highly priced and are widely used as food and medicine for the treatment of various ailments. This present review compiles literature information on the ethno-medicinal, phytochemistry and pharmacological properties of species in the genus Cordycep s. In addition, the review also suggest recommenda...
Published on Jan 1, 2018in Chemistry & Biodiversity1.45
Dan Wang1
Estimated H-index: 1
(Jiangsu University),
Jibiao Wang1
Estimated H-index: 1
(Jiangsu University)
+ 4 AuthorsYuan Wei10
Estimated H-index: 10
(Jiangsu University)
The current study was aimed at investigating the neuroprotective effects of the butanol fraction from C. cicadae (CBU), which was responsible for the anti-aging effect of this medicine. Glutamate-induced PC12 cells were used as a model to determine the neuroprotective effect against oxidative cell death. Cell viability, cytotoxicity, flow cytometry, mitochondrial transmembrane potential (MMP), reactive oxygen species (ROS), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels we...
Published on Dec 1, 2017in Phytomedicine4.18
Yanfang Sun3
Estimated H-index: 3
(ZSTU: Zhejiang Sci-Tech University),
Michael Wink66
Estimated H-index: 66
(Heidelberg University)
+ 6 AuthorsZongsuo Liang2
Estimated H-index: 2
(ZSTU: Zhejiang Sci-Tech University)
Abstract Background Cordyceps cicadae , an entomogenous fungus has been used as a dietary therapeutic in traditional Chinese medicine for several millennia, in the form of powders and decoction. However, wild C. cicadae is notably scarce. To date, there is still a lack of comprehensive and deep studies on the biological characteristics, chemical profiles and antineoplastic mechanisms of C. cicadae , especially its spores. Aim of the study This study aimed to identify wild C. cicadae using rDNA-I...
Published on Oct 2, 2017in Mycology
Yanfang Sun3
Estimated H-index: 3
(ZSTU: Zhejiang Sci-Tech University),
Yang Sun2
Estimated H-index: 2
(NU: Northeastern University)
+ 8 AuthorsLu-lu Dian1
Estimated H-index: 1
(ZSTU: Zhejiang Sci-Tech University)
ABSTRACTIsaria cicadae is an entomogenous fungus that has been used as a traditional Chinese medicinal materials to treat different diseases, including cancer. However, Isaria cicadae conidia for inhibitory activity against breast cancer cells growth are still not systematically studied. The present aim was to elucidate the phytochemical composition of Isaria cicadae conidia and to explore relevant anti-cancer potential in gynaecological carcinoma MCF-7 and Hela cells. Isaria cicadae conidia wer...
Published on Sep 25, 2017in Frontiers in Pharmacology3.85
GeoffreyBurnstock126
Estimated H-index: 126
(University of Melbourne)
Purinergic signalling, i.e. the role of nucleotides as extracellular signalling molecules, was proposed in 1972. However, this concept was not well accepted until the early 1990’s when receptor subtypes for purines and pyrimidines were cloned and characterised, which includes 4 subtypes of the P1 (adenosine) receptor, 7 subtypes of P2X ion channel receptors and 8 subtypes of the P2Y G protein-coupled receptor. Early studies were largely concerned with the physiology, pharmacology and biochemistr...
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