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Mycobacterial escape from macrophage phagosomes to the cytoplasm represents an alternate adaptation mechanism.

Published on Sep 1, 2016in Scientific Reports4.01
· DOI :10.1038/srep23089
Shilpa Jamwal6
Estimated H-index: 6
,
Parul Mehrotra2
Estimated H-index: 2
+ 3 AuthorsKanury V. S. Rao24
Estimated H-index: 24
Abstract
Survival of Mycobacterium tuberculosis (Mtb) within the host macrophage is mediated through pathogen-dependent inhibition of phagosome-lysosome fusion, which enables bacteria to persist within the immature phagosomal compartment. By employing ultrastructural examination of different field isolates supported by biochemical analysis, we found that some of the Mtb strains were in fact poorly adapted for subsistence within endocytic vesicles of infected macrophages. Instead, through a mechanism involving activation of host cytosolic phospholipase A2, these bacteria rapidly escaped from phagosomes, and established residence in the cytoplasm of the host cell. Interestingly, by facilitating an enhanced suppression of host cellular autophagy, this translocation served as an alternate virulence acquisition mechanism. Thus, our studies reveal plasticity in the adaptation strategies employed by Mtb, for survival in the host macrophage.
  • References (36)
  • Citations (34)
References36
Newest
#1Annemieke Geluk (LUMC: Leiden University Medical Center)H-Index: 32
#2Krista E. van Meijgaarden (LUMC: Leiden University Medical Center)H-Index: 35
Last.Tom H. M. Ottenhoff (LUMC: Leiden University Medical Center)H-Index: 79
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#2Alexander Rigort (MPG: Max Planck Society)H-Index: 13
Last.Wolfgang Baumeister (MPG: Max Planck Society)H-Index: 94
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#1Varshneya Singh (International Centre for Genetic Engineering and Biotechnology)H-Index: 1
#2Shilpa Jamwal (International Centre for Genetic Engineering and Biotechnology)H-Index: 6
Last.Kanury V. S. Rao (International Centre for Genetic Engineering and Biotechnology)H-Index: 24
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#1Diane Houben (NKI-AVL: Netherlands Cancer Institute)H-Index: 8
#2Caroline Demangel (Pasteur Institute)H-Index: 36
Last.Peter J. Peters (TU Delft: Delft University of Technology)H-Index: 67
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#1Roxane Simeone (Pasteur Institute)H-Index: 21
#2Alexandre Bobard (Pasteur Institute)H-Index: 7
Last.Jost Enninga (Pasteur Institute)H-Index: 25
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#2Musa A. GarbatiH-Index: 8
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#2María A. Balboa (CSIC: Spanish National Research Council)H-Index: 40
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