P009

To reduce the cost of using RosetteSepTM (RS) Human Total Lymphocyte Enrichment Cocktail (Stemcell Technologies), which improves Flow crossmatch (FC) sensitivity by removing neutrophils and monocytes. Often deceased donor blood neutrophils and monocytes are not completely removed by Ficoll Hypaque (FH) density gradient centrifugation. These cells can compete for patient HLA antibodies. Although RS can remove contaminating cells, the reagent...