LNA modification of single-stranded DNA oligonucleotides allows subtle gene modification in mismatch-repair-proficient cells

Significance With the spectacular advance of DNA-sequencing technology, a rapidly increasing number of subtle gene variants in the human population are being identified. One approach to understanding their impact on health and disease is to re-create these variants in appropriate cell lines that are accessible to functional analyses. We present a design principle for synthetic short single-stranded DNA oligonucleotides (ssODNs) that enables...