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Deletion of the single UreG urease activation gene in soybean NIL lines: characterization and pleiotropic effects

Published on Sep 1, 2016in Theoretical and Experimental Plant Physiology1.53
· DOI :10.1007/s40626-016-0052-z
Tiago Tezotto10
Estimated H-index: 10
(USP: University of São Paulo),
Sarah Caroline Ribeiro de Souza4
Estimated H-index: 4
(State University of Campinas)
+ 4 AuthorsJoseph C. Polacco36
Estimated H-index: 36
(MU: University of Missouri)
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Abstract
The soybean eu3-a mutant (formerly, eu3-e1) lacks all ureolytic activity. Eu3 encodes urease accessory (Ni insertion) protein, UreG. Eu3 (Glycine max v1.1 Glyma08g08970.1) is the only UreG-encoding gene in the soybean genome. Here we show that the eu3-a lesion is a 2.4 kb deletion, beginning 50 bp upstream of the transcription start, and covering 94 % of the deduced amino acid sequence of UreG, explaining the urease activity-null phenotype of eu3-a. We established near-isogenic lines (NILs), eu3-a versus Eu3, and confirmed a striking accumulation of endogenous urea in eu3-a and none in Eu3, and the greater resistance of eu3-a leaf discs to external urea. However, incubation of discs in light alleviated urea sensitivity in Eu3, apparently by providing C skeletons for urea-released NH3. Hence, urea, endogenous and exogenous, is apparently less toxic than urea-derived NH3. eu3-a and Eu3 plants, however, did not differ in seed Ni content, nor in pool sizes of metabolites that generate urea via arginine. The NILs differed in patterns of nodulation by Bradyrhizobium japonicum and by Bradyrhizobium elkanii. Ureases have been reported to be fungitoxic. However, eu3-a and Eu3 NIL seedlings were equally sensitive to the pathogenic Basidiomycete fungus, Rhizoctonia solani. Since eu3-a accumulates at least some apo-urease, we examined a specific anti-fungal role of the seed urease in seedlings of a second NIL pair, differing in the presence (Eu1) and absence (eu1-a) of the seed urease. Again, this second NIL pair exhibited equal sensitivity to R. solani. To our knowledge, we report only the second test of fungal resistance influenced by urease levels in intact plants.
  • References (43)
  • Citations (4)
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References43
Newest
Published on Sep 23, 2015in Frontiers in Plant Science4.11
Caio Fabiano1
Estimated H-index: 1
(State University of Campinas),
Tiago Tezotto10
Estimated H-index: 10
+ 2 AuthorsPaulo Mazzafera40
Estimated H-index: 40
(State University of Campinas)
The element Ni is considered an essential plant micronutrient because it acts as an activator of the enzyme urease. Recent studies have shown that Ni may activate an isoform of glyoxalase I, which performs an important step in the degradation of methylglyoxal (MG), a potent cytotoxic compound naturally produced by cellular metabolism. Reduced glutathione (GSH) is consumed and regenerated in the process of detoxification of MG, which is produced during stress (stress-induced production). We exami...
Published on Jun 1, 2014in Plant Journal5.73
Ananda Mustafiz8
Estimated H-index: 8
(International Centre for Genetic Engineering and Biotechnology),
Ajit Ghosh8
Estimated H-index: 8
(International Centre for Genetic Engineering and Biotechnology)
+ 7 AuthorsSneh L. Singla-Pareek11
Estimated H-index: 11
(International Centre for Genetic Engineering and Biotechnology)
†Both authors contributed equally to this work. SUMMARY The glyoxalase system constitutes the major pathway for the detoxification of metabolically produced cytotoxin methylglyoxal (MG) into a non-toxic metabolite D-lactate. Glyoxalase I (GLY I) is an evolutionarily conserved metalloenzyme requiring divalent metal ions for its activity: Zn 2+ in the case of eukaryotes or Ni 2+ for enzymes of prokaryotic origin. Plant GLY I proteins are part of a multimember family; however, not much is known abo...
Published on Jun 1, 2014in Environmental Science and Pollution Research2.91
Stefan Wernitznig4
Estimated H-index: 4
(University of Vienna),
Wolfram Adlassnig10
Estimated H-index: 10
(University of Vienna)
+ 8 AuthorsCarlo Cremisini28
Estimated H-index: 28
In the process of remediation of mine sites, the establishment of a vegetation cover is one of the most important tasks. This study tests two different approaches to manipulate soil properties in order to facilitate plant growth. Mine waste from Ingurtosu, Sardinia, Italy rich in silt, clay, and heavy metals like Cd, Cu, and Zn was used in a series of greenhouse experiments. Bacteria with putative beneficial properties for plant growth were isolated from this substrate, propagated and consortia ...
Published on Apr 23, 2014in Journal of Agricultural and Food Chemistry3.57
Mônica Medeiros-Silva2
Estimated H-index: 2
,
William L. Franck11
Estimated H-index: 11
+ 6 AuthorsCélia R. Carlini28
Estimated H-index: 28
Ureases are abundant in plants, bacteria, and in the soil, but their role in signaling between soybean and soil microorganisms has not been investigated. The bacterium Bradyrhizobium japonicum forms nitrogen-fixing nodules on soybean roots. Here, we evaluated the role(s) of ureases in the process of soybean nodulation. Chemotaxis assays demonstrated that soybean and jack bean ureases were more chemotactic toward bacterial cells than the corresponding plant lectins. The eu1-a,eu4 soybean, deficie...
Published on Mar 1, 2014in Biochimica et Biophysica Acta3.79
Anne Helene Souza Martinelli7
Estimated H-index: 7
(UFRGS: Universidade Federal do Rio Grande do Sul),
Karine Kappaun1
Estimated H-index: 1
(UFRGS: Universidade Federal do Rio Grande do Sul)
+ 10 AuthorsCristian Follmer13
Estimated H-index: 13
(UFRJ: Federal University of Rio de Janeiro)
Abstract Background Ureases are metalloenzymes involved in defense mechanisms in plants. The insecticidal activity of Canavalia ensiformis (jack bean) ureases relies partially on an internal 10 kDa peptide generated by enzymatic hydrolysis of the protein within susceptible insects. A recombinant version of this peptide, jaburetox, exhibits insecticidal, antifungal and membrane-disruptive properties. Molecular modeling of jaburetox revealed a prominent β-hairpin motif consistent with either neuro...
Published on Feb 1, 2014in Archives of Biochemistry and Biophysics3.56
Jodi L. Boer6
Estimated H-index: 6
(MSU: Michigan State University),
Scott B. Mulrooney17
Estimated H-index: 17
(MSU: Michigan State University),
Robert P. Hausinger52
Estimated H-index: 52
(MSU: Michigan State University)
This review describes the functions, structures, and mechanisms of nine nickel-containing enzymes: glyoxalase I, acireductone dioxygenase, urease, superoxide dismutase, [NiFe]-hydrogenase, carbon monoxide dehydrogenase, acetyl-coenzyme A synthase/decarbonylase, methyl-coenzyme M reductase, and lactate racemase. These enzymes catalyze their various chemistries by using metallocenters of diverse structures, including mononuclear nickel, dinuclear nickel, nickel–iron heterodinuclear sites, more com...
Published on Dec 1, 2013in Plant Physiology6.30
Devrim Coskun12
Estimated H-index: 12
(U of T: University of Toronto),
Dev T. Britto30
Estimated H-index: 30
(U of T: University of Toronto)
+ 2 AuthorsHerbert J. Kronzucker43
Estimated H-index: 43
(U of T: University of Toronto)
Futile transmembrane NH 3 /NH 4 + cycling in plant root cells, characterized by extremely rapid fluxes and high efflux to influx ratios, has been successfully linked to NH 3 /NH 4 + toxicity. Surprisingly, the fundamental question of which species of the conjugate pair (NH 3 or NH 4 + ) participates in such fluxes is unresolved. Using flux analyses with the short-lived radioisotope 13 N and electrophysiological, respiratory, and histochemical measurements, we show that futile cycling in roots of...
Published on May 1, 2013in Plant Science3.79
Joseph C. Polacco36
Estimated H-index: 36
(MU: University of Missouri),
Paulo Mazzafera40
Estimated H-index: 40
(State University of Campinas),
Tiago Tezotto10
Estimated H-index: 10
(ESALQ: Escola Superior de Agricultura Luiz de Queiroz)
University of Missouri, Department of Biochemistry, Interdisciplinary Plant Group, 117 Schweitzer Hall, Columbia, MO 65211, United States Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Biologia Vegetal, CP 6109, 13083-970 Campinas, SP, Brazil Universidade de Sao Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Produc ao Vegetal, CP 9, 13418-900 Piracicaba, SP, Brazil
Published on Mar 1, 2013in Canadian Journal of Plant Science0.99
ZhangJ. X.1
Estimated H-index: 1
,
XueA. G.2
Estimated H-index: 2
+ 4 AuthorsGregorichE.1
Estimated H-index: 1
Zhang, J. X., Xue, A. G., Cober, E. R., Morrison, M. J., Zhang, H. J., Zhang, S. Z. and Gregorich, E. 2013. Prevalence, pathogenicity and cultivar resistance of Fusarium and Rhizoctonia species causing soybean root rot. Can. J. Plant Sci. 93: 221–236. Root rot complex, caused by Fusarium and Rhizoctonia species, is a major soybean disease in Canada. We isolated nine Fusarium and Rhizoctonia species including F. oxysporum (Fo), F. graminearum (Fg), F. solani (Fs), F. avenaceum (Fa), F. tricinctum...
Published on Feb 1, 2013in Plant and Soil3.26
Bahar Yildiz Kutman5
Estimated H-index: 5
(Sabancı University),
Umit Baris Kutman9
Estimated H-index: 9
(Sabancı University),
Ismail Cakmak67
Estimated H-index: 67
(Sabancı University)
Background and aims The importance of seed Ni reserves for plant growth and N metabolism is poorly understood. This study investigated the effects of both seed Ni and externally supplied Ni on the impact of foliarly-applied urea and N-nutritional status of soybean.
Cited By4
Newest
Published on May 8, 2018in Frontiers in Plant Science4.11
Douglas Siqueira Freitas2
Estimated H-index: 2
,
Bruna Wurr Rodak2
Estimated H-index: 2
+ 5 AuthorsLuiz Roberto Guimarães Guilherme22
Estimated H-index: 22
Nickel (Ni) - a component of urease and hydrogenase - was the latest nutrient to be included in the list of essential elements to plants. Although relevant studies have been performed concerning its essentiality, there are no records of Ni deficiency for annual species cultivated under field conditions. The reason might be the nonappearance of obvious symptoms, i.e., a hidden (or latent) deficiency. Soybean, a crop cultivated on soils poor in available Ni content, has a high dependence on biolog...
Published on Mar 2, 2017in Genetics and Molecular Biology2.13
Ciliana Rechenmacher4
Estimated H-index: 4
(UFRGS: Universidade Federal do Rio Grande do Sul),
Beatriz Wiebke-Strohm7
Estimated H-index: 7
(UFRGS: Universidade Federal do Rio Grande do Sul)
+ 3 AuthorsMaria Helena Bodanese-Zanettini20
Estimated H-index: 20
(UFRGS: Universidade Federal do Rio Grande do Sul)
Abstract Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product ofurease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as inprimary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by theEu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, desig-nated Eu5, which encodes the putative prote...
Published on Feb 1, 2017in Process Biochemistry2.88
Anne Helene Souza Martinelli7
Estimated H-index: 7
(UFRGS: Universidade Federal do Rio Grande do Sul),
Fernanda Cortez Lopes8
Estimated H-index: 8
(PUCRS: Pontifícia Universidade Católica do Rio Grande do Sul)
+ 12 AuthorsDiogo Ribeiro Demartini9
Estimated H-index: 9
(UFRGS: Universidade Federal do Rio Grande do Sul)
Abstract Ureases are nickel-dependent enzymes that catalyze the hydrolysis of urea to ammonia and carbon dioxide. In soybean ( Glycine max ), the embryo-specific urease (eSBU), the ubiquitous urease (uSBU), and a third isoform (SBU-III) are synthesized. Our group has previously demonstrated that eSBU, purified from seeds, has antifungal properties against phytopathogenic fungi, entomotoxicity against Dysdercus peruvianus , the ability to induce blood platelet aggregation, and these properties ar...
Published on Jan 1, 2016
J. P. Q. Barcelos3
Estimated H-index: 3
,
E. Furlani Junior1
Estimated H-index: 1
+ 2 AuthorsA. R. dos Reis1
Estimated H-index: 1
Nitrogen is the most required nutrient by cotton plants, and its storage form in plants involves ureides and arginine, which makes nickel (component metalloenzyme urease) essential for metabolism and efficiency of nitrogen utilization by cotton plants. This study aimed to evaluate the response of cotton cv. FMT-701 due to combination of nitrogen and
View next paperMutational analysis of the embryo-specific urease locus of soybean