Inhibitory effects of S-allylmercaptocysteine against benzo(a)pyrene-induced precancerous carcinogenesis in human lung cells

Published on May 1, 2016in International Immunopharmacology3.361
· DOI :10.1016/j.intimp.2016.02.017
Kaiming Wang6
Estimated H-index: 6
(SDU: Shandong University),
Ying Wang4
Estimated H-index: 4
(SDU: Shandong University)
+ 9 AuthorsShanzhong Li6
Estimated H-index: 6
Abstract The anti -cancer effects of oil-soluble organosulfur compounds in garlic in the initiation phase of carcinogenesis are known. However, there are few experimental studies investigating S -allylmercaptocysteine (SAMC), a water-soluble derivative of garlic. This study investigated whether SAMC prevented the carcinogen benzo(a)pyrene (B(a)P) from inducing precancerous activity in human lung cells (A549 cell line). A549 cells were either pre-treated (PreTM) or concurrently treated (CoTM) with 1 μM B(a)P and either 10 or 50 μM SAMC. The 50 μM PreTM group inhibited B(a)P-induced cell proliferation by approximately 100%. The 50 μM SAMC PreTM and CoTM inhibited the B(a)P-induced G2/M phase shift by 100% and 97%, respectively. Furthermore, the PreTM and CoTM groups exhibited the potential to reduce the generation of reactive oxygen species (ROS) relative to the B(a)P group by at least 78%. The SAMC PreTM elevated superoxide dismutase (SOD) by approximately 100%. In this study, we revealed the mechanisms involved in SAMC inhibition of B(a)P-induced carcinogenesis, including suppression of cell proliferation, cell cycle regulation, attenuation of ROS formation, inhibition of DNA damage, increase of SOD activity and inhibition of nuclear factor-kappa B (NF-κB) activity. SAMC appears to be a novel therapeutic candidate for the prevention and treatment of B(a)P-induced human lung cancer.
  • References (49)
  • Citations (6)
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