Determining performance characteristics of an NGS ‐based HLA typing method for clinical applications
Abstract
This study presents performance specifications of an in-house developed human leukocyte antigen (HLA) typing assay using next-generation sequencing (NGS) on the Illumina MiSeq platform. A total of 253 samples, previously characterized for HLA-A, -B, -C, -DRB1 and -DQB1 were included in this study, which were typed at high-resolution using a combination of Sanger sequencing, sequence-specific primer (SSP) and sequence-specific oligonucleotide...
Paper Details
Title
Determining performance characteristics of an NGS ‐based HLA typing method for clinical applications
Published Date
Feb 16, 2016
Volume
87
Issue
3
Pages
141 - 152
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