Crystallization of Membrane Proteins by Vapor Diffusion
Abstract
X-ray crystallography remains the most robust method to determine protein structure at the atomic level. However, the bottlenecks of protein expression and purification often discourage further study. In this chapter, we address the most common problems encountered at these stages. Based on our experiences in expressing and purifying antimicrobial efflux proteins, we explain how a pure and homogenous protein sample can be successfully...
Paper Details
Title
Crystallization of Membrane Proteins by Vapor Diffusion
Published Date
Jan 1, 2015
Journal
Volume
557
Pages
363 - 392
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