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Biochemical profiling of proteins and metabolites in wound exudate from chronic wound environments

Published on Jan 1, 2012
Daniel Broszczak4
Estimated H-index: 4
,
Dario Stupar2
Estimated H-index: 2
+ 5 AuthorsMelissa Laura Fernandez4
Estimated H-index: 4
Abstract
The lack of fundamental knowledge on the biological processes associated with wound healing represents a significant challenge. Understanding the biochemical changes that occur within a chronic wound could provide insights into the wound environment and enable more effective wound management. We report on the stability of wound fluid samples under various conditions and describe a high-throughput approach to investigate the altered biochemical state within wound samples collected from various types of chronic, ulcerated wounds. Furthermore, we discuss the viability of this approach in the early stages of wound sample protein and metabolite profiling and subsequent biomarker discovery. This approach will facilitate the detection of factors that may correlate with wound severity and/or could be used to monitor the response to a particular treatment.
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  • Citations (6)
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Cited By6
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Published on Jan 1, 2017
Q. Zeng1
Estimated H-index: 1
(SUNY: State University of New York System),
L.K. Macri1
Estimated H-index: 1
(SUNY: State University of New York System)
+ 10 AuthorsDario Stupar2
Estimated H-index: 2
The integration of healing, cell biology, and skin tissue engineering research has been ongoing for nearly one century. In this chapter, we provide a bird’s eye view of skin anatomy and functions, wound healing processes, the challenges and solutions to wound healing. Many techniques and biomaterials have been examined for their potential utility as skin substitutes. Notwithstanding evidence that some strategies have been more successful than others, the ideal skin substitute does not exist. Exi...
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Published on Jan 1, 2016
Steven J. P. McInnes13
Estimated H-index: 13
,
Christopher T. Turner7
Estimated H-index: 7
+ 1 AuthorsH VoelckerNicolas45
Estimated H-index: 45
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Published on Dec 1, 2015in British Journal of Dermatology 6.71
Doris Schiffer3
Estimated H-index: 3
,
Miriam H.E. Blokhuis-Arkes3
Estimated H-index: 3
(Medisch Spectrum Twente)
+ 3 AuthorsGeorg Gübitz58
Estimated H-index: 58
(BOKU: University of Natural Resources and Life Sciences, Vienna)
Infection in wounds affects about 2% of the population in developed countires at least once in their lifetime, and the lack of tools for its rapid diagnosis is still a problem 1. Standard procedures of infection detection include the judgement of the classical clinical signs, the detection of signals specific to secondary wounds, or the quantification of the microbial load 2–5. The determination of the microbial load is a time-consuming standard procedure, although the presence of microbes per s...
6 Citations Source Cite
Published on Jun 1, 2015in Applied Microbiology and Biotechnology 3.67
Gregor Tegl6
Estimated H-index: 6
(BOKU: University of Natural Resources and Life Sciences, Vienna),
Doris Schiffer3
Estimated H-index: 3
(BOKU: University of Natural Resources and Life Sciences, Vienna)
+ 2 AuthorsGeorg Gübitz58
Estimated H-index: 58
(BOKU: University of Natural Resources and Life Sciences, Vienna)
Wound infection is a severe complication causing delayed healing and risks for patients. Conventional methods of diagnosis for infection involve error-prone clinical description of the wound and time-consuming microbiological tests. More reliable alternatives are still rare, except for invasive and unaffordable gold standard methods. This review discusses the diversity of new approaches for wound infection determination. There has been progress in the detection methods of microorganisms, includi...
12 Citations Source Cite
Published on May 1, 2015in Engineering in Life Sciences 1.94
Doris Schiffer3
Estimated H-index: 3
,
Vanessa Verient2
Estimated H-index: 2
+ 7 AuthorsGeorg Gübitz58
Estimated H-index: 58
(BOKU: University of Natural Resources and Life Sciences, Vienna)
There is a strong need for new point-of-care systems for the detection of wound infection. Overseen infections in chronic wounds induce severe complications, such as delayed healing and high risks for the patients, while time-consuming common gold and silver standard methods for infection assessment cannot be implemented in home care units. This study demonstrates for the first time the between correlation of lysozyme activity and silver-standard microbiological evaluation of wounds. Significant...
4 Citations Source Cite